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High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil

BACKGROUND: Botanical substances such as essential oils (EOs) have demonstrated insecticidal properties and are a valid option for vector control. However, free EOs are unreliable as mosquito larvicides due their easy degradation by environmental exposure to ultraviolet light and higher temperatures...

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Autores principales: Gomes, Bruno, Ogélio, Huarlen, Brant, Fabiane, Pereira-Pinto, Camila Jesus, Workman, Michael J., Costa, Monique, Lima, José Bento Pereira, Martins, Ademir Jesus, Ramalho-Ortigao, Marcelo, Durvasula, Ravi, Hurwitz, Ivy, David, Mariana Rocha, Genta, Fernando Ariel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140510/
https://www.ncbi.nlm.nih.gov/pubmed/34022935
http://dx.doi.org/10.1186/s13071-021-04733-2
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author Gomes, Bruno
Ogélio, Huarlen
Brant, Fabiane
Pereira-Pinto, Camila Jesus
Workman, Michael J.
Costa, Monique
Lima, José Bento Pereira
Martins, Ademir Jesus
Ramalho-Ortigao, Marcelo
Durvasula, Ravi
Hurwitz, Ivy
David, Mariana Rocha
Genta, Fernando Ariel
author_facet Gomes, Bruno
Ogélio, Huarlen
Brant, Fabiane
Pereira-Pinto, Camila Jesus
Workman, Michael J.
Costa, Monique
Lima, José Bento Pereira
Martins, Ademir Jesus
Ramalho-Ortigao, Marcelo
Durvasula, Ravi
Hurwitz, Ivy
David, Mariana Rocha
Genta, Fernando Ariel
author_sort Gomes, Bruno
collection PubMed
description BACKGROUND: Botanical substances such as essential oils (EOs) have demonstrated insecticidal properties and are a valid option for vector control. However, free EOs are unreliable as mosquito larvicides due their easy degradation by environmental exposure to ultraviolet light and higher temperatures. Here, we assessed the efficacy of a mosquito larvicide based on orange oil in a yeast-based delivery system against Aedes aegypti strains with different resistance status towards chemical neurotoxic insecticides. This larvicide preparation was physicochemically characterized in a previous report. METHODS: Larvae of four Ae. aegypti strains from different regions of Brazil and different resistance profiles for deltamethrin (pyrethroid) and temephos (organophosphate) were tested against yeast-encapsulated orange oil (YEOO) in laboratory conditions for measurement of LC(50) and LC(90) values. The same assays were performed with the Belo Horizonte strain under environmental conditions (natural light and temperature). The resistance profiles of these strains were compared to the Rockefeller reference strain in all conditions. RESULTS: YEOO was found to be a highly active larvicide (LC(50) < 50 mg/L) against all Ae. aegypti strains tested in both laboratory conditions (LC(50) = 8.1–24.7 mg/L) and environmental conditions with natural light and temperature fluctuation (LC(50) = 20.0–49.9 mg/L). Moreover, all strains were considered susceptible (RR < 5) to YEOO, considering resistance ratios calculated based on the Rockefeller strain. The resistance ratios were only higher than 2.5 for LC(90–95) of Belo Horizonte in the laboratory, probably due the higher heterogeneity associated with older egg papers (> 5 months). CONCLUSION: YEOO demonstrates high larvicidal activity against Ae. aegypti strains with resistant phenotypes for deltamethrin (PY) and temephos (OP). This larvicidal activity suggests the potential for the development of YEOO as an alternative intervention to synthetic insecticides in integrated vector management programs, for populations with resistance to commonly used insecticides. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-021-04733-2.
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spelling pubmed-81405102021-05-25 High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil Gomes, Bruno Ogélio, Huarlen Brant, Fabiane Pereira-Pinto, Camila Jesus Workman, Michael J. Costa, Monique Lima, José Bento Pereira Martins, Ademir Jesus Ramalho-Ortigao, Marcelo Durvasula, Ravi Hurwitz, Ivy David, Mariana Rocha Genta, Fernando Ariel Parasit Vectors Research BACKGROUND: Botanical substances such as essential oils (EOs) have demonstrated insecticidal properties and are a valid option for vector control. However, free EOs are unreliable as mosquito larvicides due their easy degradation by environmental exposure to ultraviolet light and higher temperatures. Here, we assessed the efficacy of a mosquito larvicide based on orange oil in a yeast-based delivery system against Aedes aegypti strains with different resistance status towards chemical neurotoxic insecticides. This larvicide preparation was physicochemically characterized in a previous report. METHODS: Larvae of four Ae. aegypti strains from different regions of Brazil and different resistance profiles for deltamethrin (pyrethroid) and temephos (organophosphate) were tested against yeast-encapsulated orange oil (YEOO) in laboratory conditions for measurement of LC(50) and LC(90) values. The same assays were performed with the Belo Horizonte strain under environmental conditions (natural light and temperature). The resistance profiles of these strains were compared to the Rockefeller reference strain in all conditions. RESULTS: YEOO was found to be a highly active larvicide (LC(50) < 50 mg/L) against all Ae. aegypti strains tested in both laboratory conditions (LC(50) = 8.1–24.7 mg/L) and environmental conditions with natural light and temperature fluctuation (LC(50) = 20.0–49.9 mg/L). Moreover, all strains were considered susceptible (RR < 5) to YEOO, considering resistance ratios calculated based on the Rockefeller strain. The resistance ratios were only higher than 2.5 for LC(90–95) of Belo Horizonte in the laboratory, probably due the higher heterogeneity associated with older egg papers (> 5 months). CONCLUSION: YEOO demonstrates high larvicidal activity against Ae. aegypti strains with resistant phenotypes for deltamethrin (PY) and temephos (OP). This larvicidal activity suggests the potential for the development of YEOO as an alternative intervention to synthetic insecticides in integrated vector management programs, for populations with resistance to commonly used insecticides. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-021-04733-2. BioMed Central 2021-05-22 /pmc/articles/PMC8140510/ /pubmed/34022935 http://dx.doi.org/10.1186/s13071-021-04733-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Gomes, Bruno
Ogélio, Huarlen
Brant, Fabiane
Pereira-Pinto, Camila Jesus
Workman, Michael J.
Costa, Monique
Lima, José Bento Pereira
Martins, Ademir Jesus
Ramalho-Ortigao, Marcelo
Durvasula, Ravi
Hurwitz, Ivy
David, Mariana Rocha
Genta, Fernando Ariel
High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil
title High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil
title_full High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil
title_fullStr High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil
title_full_unstemmed High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil
title_short High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil
title_sort high larvicidal efficacy of yeast-encapsulated orange oil against aedes aegypti strains from brazil
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140510/
https://www.ncbi.nlm.nih.gov/pubmed/34022935
http://dx.doi.org/10.1186/s13071-021-04733-2
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