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Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets

Proteins are considered the most expensive nutrients in commercial modern broiler production, and their dietary inclusion at low levels is pivotal to minimize feed costs and reduce nitrogen waste. The quest for an environmentally friendly source of proteins that favor the formulation of low protein...

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Autores principales: Mullenix, Garrett J., Greene, Elizabeth S., Emami, Nima K., Tellez-Isaias, Guillermo, Bottje, Walter G., Erf, Gisela F., Kidd, Michael T., Dridi, Sami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141556/
https://www.ncbi.nlm.nih.gov/pubmed/34041289
http://dx.doi.org/10.3389/fvets.2021.640968
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author Mullenix, Garrett J.
Greene, Elizabeth S.
Emami, Nima K.
Tellez-Isaias, Guillermo
Bottje, Walter G.
Erf, Gisela F.
Kidd, Michael T.
Dridi, Sami
author_facet Mullenix, Garrett J.
Greene, Elizabeth S.
Emami, Nima K.
Tellez-Isaias, Guillermo
Bottje, Walter G.
Erf, Gisela F.
Kidd, Michael T.
Dridi, Sami
author_sort Mullenix, Garrett J.
collection PubMed
description Proteins are considered the most expensive nutrients in commercial modern broiler production, and their dietary inclusion at low levels is pivotal to minimize feed costs and reduce nitrogen waste. The quest for an environmentally friendly source of proteins that favor the formulation of low protein diets without compromising broiler health, welfare, and growth performance has become a hotspot in nutrition research. Due to its high protein content, the naturally growing Spirulina microalgae is considered a promising nutrient source. The purpose of the present study was, therefore, to determine the effects of Spirulina supplementation on liver bacterial translocation, hematological profile, and circulating inflammatory and redox markers in broilers fed a low-protein diet. One-day-old Ross 708 male broilers (n = 180) were randomly assigned into one of three experimental treatments: standard diet as a control, low protein diet, and low protein diet supplemented with 100 g/kg of Spirulina. Target molecular markers were measured in the peripheral blood circulation using real-time quantitative PCR. Reducing dietary proteins increased bacterial translocation and systemic inflammation as indicated by proportions of basophils among blood leukocytes. The expression levels of circulating pro-inflammatory cytokines [interleukin (IL)-3, IL-6, IL-4, IL-18, and tumor necrosis factor-α], chemokines (CCL-20), and NOD-like receptor family pyrin domain containing 3 inflammasome were significantly upregulated in birds fed the low protein diet compared with the control. The inclusion of Spirulina reversed these effects, which indicates that Spirulina reduces systemic inflammation- and bacterial translocation-induced by a low protein diet and could be a promising alternative protein source in poultry diets.
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spelling pubmed-81415562021-05-25 Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets Mullenix, Garrett J. Greene, Elizabeth S. Emami, Nima K. Tellez-Isaias, Guillermo Bottje, Walter G. Erf, Gisela F. Kidd, Michael T. Dridi, Sami Front Vet Sci Veterinary Science Proteins are considered the most expensive nutrients in commercial modern broiler production, and their dietary inclusion at low levels is pivotal to minimize feed costs and reduce nitrogen waste. The quest for an environmentally friendly source of proteins that favor the formulation of low protein diets without compromising broiler health, welfare, and growth performance has become a hotspot in nutrition research. Due to its high protein content, the naturally growing Spirulina microalgae is considered a promising nutrient source. The purpose of the present study was, therefore, to determine the effects of Spirulina supplementation on liver bacterial translocation, hematological profile, and circulating inflammatory and redox markers in broilers fed a low-protein diet. One-day-old Ross 708 male broilers (n = 180) were randomly assigned into one of three experimental treatments: standard diet as a control, low protein diet, and low protein diet supplemented with 100 g/kg of Spirulina. Target molecular markers were measured in the peripheral blood circulation using real-time quantitative PCR. Reducing dietary proteins increased bacterial translocation and systemic inflammation as indicated by proportions of basophils among blood leukocytes. The expression levels of circulating pro-inflammatory cytokines [interleukin (IL)-3, IL-6, IL-4, IL-18, and tumor necrosis factor-α], chemokines (CCL-20), and NOD-like receptor family pyrin domain containing 3 inflammasome were significantly upregulated in birds fed the low protein diet compared with the control. The inclusion of Spirulina reversed these effects, which indicates that Spirulina reduces systemic inflammation- and bacterial translocation-induced by a low protein diet and could be a promising alternative protein source in poultry diets. Frontiers Media S.A. 2021-05-10 /pmc/articles/PMC8141556/ /pubmed/34041289 http://dx.doi.org/10.3389/fvets.2021.640968 Text en Copyright © 2021 Mullenix, Greene, Emami, Tellez-Isaias, Bottje, Erf, Kidd and Dridi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Mullenix, Garrett J.
Greene, Elizabeth S.
Emami, Nima K.
Tellez-Isaias, Guillermo
Bottje, Walter G.
Erf, Gisela F.
Kidd, Michael T.
Dridi, Sami
Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets
title Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets
title_full Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets
title_fullStr Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets
title_full_unstemmed Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets
title_short Spirulina platensis Inclusion Reverses Circulating Pro-inflammatory (Chemo)cytokine Profiles in Broilers Fed Low-Protein Diets
title_sort spirulina platensis inclusion reverses circulating pro-inflammatory (chemo)cytokine profiles in broilers fed low-protein diets
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141556/
https://www.ncbi.nlm.nih.gov/pubmed/34041289
http://dx.doi.org/10.3389/fvets.2021.640968
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