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A Fast and Efficient Single-stranded Genomic Library Preparation Method Optimized for Ancient DNA

We present a protocol to prepare extracted DNA for sequencing on the Illumina sequencing platform that has been optimized for ancient and degraded DNA. Our approach, the Santa Cruz Reaction or SCR, uses directional splinted ligation of Illumina’s P5 and P7 adapters to convert natively single-strande...

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Detalles Bibliográficos
Autores principales:	Kapp, Joshua D, Green, Richard E, Shapiro, Beth
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141684/ https://www.ncbi.nlm.nih.gov/pubmed/33768239 http://dx.doi.org/10.1093/jhered/esab012

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