Cargando…
In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle
Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both Chlamydia trachomatis and Chlamydia pneumoniae can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141889/ https://www.ncbi.nlm.nih.gov/pubmed/34041379 http://dx.doi.org/10.1016/j.heliyon.2021.e06947 |
_version_ | 1783696462748581888 |
---|---|
author | Entrocassi, Andrea Carolina Catalano, Alejandra Vanina Ouviña, Adriana Graciela Wilson, Erica Georgina López, Paula Gladys Rodríguez Fermepin, Marcelo |
author_facet | Entrocassi, Andrea Carolina Catalano, Alejandra Vanina Ouviña, Adriana Graciela Wilson, Erica Georgina López, Paula Gladys Rodríguez Fermepin, Marcelo |
author_sort | Entrocassi, Andrea Carolina |
collection | PubMed |
description | Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both Chlamydia trachomatis and Chlamydia pneumoniae can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method in order to detect in vitro antichlamydial activity of different extracts obtained from native Argentinian plants used as antimicrobials in local ethnomedicine and to evaluate their inhibitory activity over Chlamydia trachomatis and Chlamydia pneumoniae growth. The inhibitory activity over different stages of the chlamydial life cycle on cell culture was assessed: the entry, the inclusion developing after entry, and the exponential growth stage. Also, the capability of rendering the cell refractory to chlamydial infection by pre-incubation with the extracts was assayed. Inhibitory activity of water-based and organic-based extracts obtained from Hydrocotyle bonariensis Lam. (Araliaceae), Lithraea molleoides (Vell.) Engl. (Anacardiaceae) and Hybanthus parviflorus (Mutis ex L.f.) Baill. (Violaceae) were tested against five strains of Chlamydia trachomatis (L2/434/BU and four clinical isolates form both neonatal conjunctivitis and adult genital infections, genotypes D, E, and K) and against Chlamydia pneumoniae AR39. The Hydrocotyle bonariensis dichloromethane extract showed a broad inhibitory activity over the exponential growth stage of Chlamydia trachomatis and Chlamydia pneumoniae independently from the chlamydial strain and the cell line. These results suggest a high inhibitory potential on both Chlamydiae species. In order to characterize the Hydrocotyle bonariensis dichloromethane active extract, an (1)H-NMR was performed. The (1)H-NMR characterization showed a spectrum with characteristic signals of the fatty acid moiety of lipids or cerebrosides, volatile phenolics, phytosterols, methyl triterpenes signals, and glucose moiety of the cerebrosides. |
format | Online Article Text |
id | pubmed-8141889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81418892021-05-25 In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle Entrocassi, Andrea Carolina Catalano, Alejandra Vanina Ouviña, Adriana Graciela Wilson, Erica Georgina López, Paula Gladys Rodríguez Fermepin, Marcelo Heliyon Research Article Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both Chlamydia trachomatis and Chlamydia pneumoniae can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method in order to detect in vitro antichlamydial activity of different extracts obtained from native Argentinian plants used as antimicrobials in local ethnomedicine and to evaluate their inhibitory activity over Chlamydia trachomatis and Chlamydia pneumoniae growth. The inhibitory activity over different stages of the chlamydial life cycle on cell culture was assessed: the entry, the inclusion developing after entry, and the exponential growth stage. Also, the capability of rendering the cell refractory to chlamydial infection by pre-incubation with the extracts was assayed. Inhibitory activity of water-based and organic-based extracts obtained from Hydrocotyle bonariensis Lam. (Araliaceae), Lithraea molleoides (Vell.) Engl. (Anacardiaceae) and Hybanthus parviflorus (Mutis ex L.f.) Baill. (Violaceae) were tested against five strains of Chlamydia trachomatis (L2/434/BU and four clinical isolates form both neonatal conjunctivitis and adult genital infections, genotypes D, E, and K) and against Chlamydia pneumoniae AR39. The Hydrocotyle bonariensis dichloromethane extract showed a broad inhibitory activity over the exponential growth stage of Chlamydia trachomatis and Chlamydia pneumoniae independently from the chlamydial strain and the cell line. These results suggest a high inhibitory potential on both Chlamydiae species. In order to characterize the Hydrocotyle bonariensis dichloromethane active extract, an (1)H-NMR was performed. The (1)H-NMR characterization showed a spectrum with characteristic signals of the fatty acid moiety of lipids or cerebrosides, volatile phenolics, phytosterols, methyl triterpenes signals, and glucose moiety of the cerebrosides. Elsevier 2021-05-18 /pmc/articles/PMC8141889/ /pubmed/34041379 http://dx.doi.org/10.1016/j.heliyon.2021.e06947 Text en © 2021 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Entrocassi, Andrea Carolina Catalano, Alejandra Vanina Ouviña, Adriana Graciela Wilson, Erica Georgina López, Paula Gladys Rodríguez Fermepin, Marcelo In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle |
title | In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle |
title_full | In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle |
title_fullStr | In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle |
title_full_unstemmed | In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle |
title_short | In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle |
title_sort | in vitro inhibitory effect of hydrocotyle bonariensis lam. extracts over chlamydia trachomatis and chlamydia pneumoniae on different stages of the chlamydial life cycle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141889/ https://www.ncbi.nlm.nih.gov/pubmed/34041379 http://dx.doi.org/10.1016/j.heliyon.2021.e06947 |
work_keys_str_mv | AT entrocassiandreacarolina invitroinhibitoryeffectofhydrocotylebonariensislamextractsoverchlamydiatrachomatisandchlamydiapneumoniaeondifferentstagesofthechlamydiallifecycle AT catalanoalejandravanina invitroinhibitoryeffectofhydrocotylebonariensislamextractsoverchlamydiatrachomatisandchlamydiapneumoniaeondifferentstagesofthechlamydiallifecycle AT ouvinaadrianagraciela invitroinhibitoryeffectofhydrocotylebonariensislamextractsoverchlamydiatrachomatisandchlamydiapneumoniaeondifferentstagesofthechlamydiallifecycle AT wilsonericageorgina invitroinhibitoryeffectofhydrocotylebonariensislamextractsoverchlamydiatrachomatisandchlamydiapneumoniaeondifferentstagesofthechlamydiallifecycle AT lopezpaulagladys invitroinhibitoryeffectofhydrocotylebonariensislamextractsoverchlamydiatrachomatisandchlamydiapneumoniaeondifferentstagesofthechlamydiallifecycle AT rodriguezfermepinmarcelo invitroinhibitoryeffectofhydrocotylebonariensislamextractsoverchlamydiatrachomatisandchlamydiapneumoniaeondifferentstagesofthechlamydiallifecycle |