Cargando…
Immunofluorescence microscopy-based assessment of cytosolic DNA accumulation in mammalian cells
Here, we describe an immunofluorescence (IF) microscopy-based approach to quantify cytosolic double-stranded DNA molecules in cultured eukaryotic cells upon the selective and specific permeabilization of plasma membranes. This technique is compatible with widefield microscopy coupled with automated...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141942/ https://www.ncbi.nlm.nih.gov/pubmed/34041502 http://dx.doi.org/10.1016/j.xpro.2021.100488 |