A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets

Transcriptional changes happen within minutes; however, RNAi or genetic deletion requires days to weeks before transcription networks can be analyzed. This limitation has made it challenging to distinguish direct from indirect targets of sequence-specific transcription factors. This inability to def...

Descripción completa

Detalles Bibliográficos
Autores principales: Layden, Hillary M., Eleuteri, Nicholas A., Hiebert, Scott W., Stengel, Kristy R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142277/
https://www.ncbi.nlm.nih.gov/pubmed/34041503
http://dx.doi.org/10.1016/j.xpro.2021.100530

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142277/
https://www.ncbi.nlm.nih.gov/pubmed/34041503
http://dx.doi.org/10.1016/j.xpro.2021.100530

Ejemplares similares