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Development and validation of portable, field-deployable Ebola virus point-of-encounter diagnostic assay for wildlife surveillance

ABSTRACT: Early detection of Ebola virus spillover into wildlife is crucial for rapid response. We developed and validated a portable, cold-chain independent Ebola virus RT-qPCR assay. METHODS: The field syringe-based RNA extraction method was compared with a conventional laboratory-based spin-colum...

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Detalles Bibliográficos
Autores principales: Figueroa, Dania M., Kuisma, Eeva, Matson, M. Jeremiah, Ondzie, Alain U., Bushmaker, Trent, Seifert, Stephanie N., Ntoumi, Francine, Escudero-Pérez, Beatriz, Muñoz-Fontela, César, Walzer, Chris, Olson, Sarah H., Goma-Nkoua, Cynthia, Mombouli, Jean-Vivien, Fischer, Robert J., Munster, Vincent J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142476/
https://www.ncbi.nlm.nih.gov/pubmed/34024280
http://dx.doi.org/10.1186/s42522-021-00041-y