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Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection

HEV-Ag ELISA assay is a reliable diagnostic test in resource-limited areas. HEV genotype 1 (HEV-1) infections are either self-limited or progress to fulminant hepatic failure (FHF) and death if anti-HEV therapy is delayed. Limited data is available about the diagnostic utility of HEV Ag on HEV-1 inf...

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Autores principales: El-Mokhtar, Mohamed A., Karam-Allah Ramadan, Haidi, Abdel Hameed, Muhamad R., M. Kamel, Ayat, A. Mandour, Sahar, Ali, Maha, Abdel-Malek, Mohamed A. Y., M. Abd El-Kareem, Doaa, Adel, Sara, H. Salama, Eman, Khalaf, Khaled Abo Bakr, Sayed, Ibrahim M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143225/
https://www.ncbi.nlm.nih.gov/pubmed/34002677
http://dx.doi.org/10.1080/21505594.2021.1922027
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author El-Mokhtar, Mohamed A.
Karam-Allah Ramadan, Haidi
Abdel Hameed, Muhamad R.
M. Kamel, Ayat
A. Mandour, Sahar
Ali, Maha
Abdel-Malek, Mohamed A. Y.
M. Abd El-Kareem, Doaa
Adel, Sara
H. Salama, Eman
Khalaf, Khaled Abo Bakr
Sayed, Ibrahim M.
author_facet El-Mokhtar, Mohamed A.
Karam-Allah Ramadan, Haidi
Abdel Hameed, Muhamad R.
M. Kamel, Ayat
A. Mandour, Sahar
Ali, Maha
Abdel-Malek, Mohamed A. Y.
M. Abd El-Kareem, Doaa
Adel, Sara
H. Salama, Eman
Khalaf, Khaled Abo Bakr
Sayed, Ibrahim M.
author_sort El-Mokhtar, Mohamed A.
collection PubMed
description HEV-Ag ELISA assay is a reliable diagnostic test in resource-limited areas. HEV genotype 1 (HEV-1) infections are either self-limited or progress to fulminant hepatic failure (FHF) and death if anti-HEV therapy is delayed. Limited data is available about the diagnostic utility of HEV Ag on HEV-1 infections. Herein wWe aimed to study the kinetics of HEV Ag during HEV-1 infections at different stages, i.e., acute HEV infection, recovery, and progression to FHF. Also, we evaluated the diagnostic utility of this marker to predict the outcomes of HEV-1 infections. Plasma of acute hepatitis E (AHE) patients were assessed for HEV RNA by RT-qPCR, HEV Ag, and anti-HEV IgM by ELISA. The kinetics of HEV Ag was monitored at different time points; acute phase of infection, recovery, FHF stage, and post-recovery. Our results showed that the level of HEV Ag was elevated in AHE patients with a significantly higher level in FHF patients than recovered patients. We identified a plasma HEV Ag threshold that can differentiate between self-limiting infection and FHF progression with 100% sensitivity and 88.89% specificity. HEV Ag and HEV RNA have similar kinetics during the acute phase and self-limiting infection. In the FHF stage, HEV Ag and anti-HEV IgM have similar patterns of kinetics which could be the cause of liver damage. In conclusion, the HEV Ag assay can be used as a biomarker for predicting the consequences of HEV-1 infections which could be diagnostically useful for taking the appropriate measures to reduce the complications, especially for high-risk groups.
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spelling pubmed-81432252021-06-07 Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection El-Mokhtar, Mohamed A. Karam-Allah Ramadan, Haidi Abdel Hameed, Muhamad R. M. Kamel, Ayat A. Mandour, Sahar Ali, Maha Abdel-Malek, Mohamed A. Y. M. Abd El-Kareem, Doaa Adel, Sara H. Salama, Eman Khalaf, Khaled Abo Bakr Sayed, Ibrahim M. Virulence Research Paper HEV-Ag ELISA assay is a reliable diagnostic test in resource-limited areas. HEV genotype 1 (HEV-1) infections are either self-limited or progress to fulminant hepatic failure (FHF) and death if anti-HEV therapy is delayed. Limited data is available about the diagnostic utility of HEV Ag on HEV-1 infections. Herein wWe aimed to study the kinetics of HEV Ag during HEV-1 infections at different stages, i.e., acute HEV infection, recovery, and progression to FHF. Also, we evaluated the diagnostic utility of this marker to predict the outcomes of HEV-1 infections. Plasma of acute hepatitis E (AHE) patients were assessed for HEV RNA by RT-qPCR, HEV Ag, and anti-HEV IgM by ELISA. The kinetics of HEV Ag was monitored at different time points; acute phase of infection, recovery, FHF stage, and post-recovery. Our results showed that the level of HEV Ag was elevated in AHE patients with a significantly higher level in FHF patients than recovered patients. We identified a plasma HEV Ag threshold that can differentiate between self-limiting infection and FHF progression with 100% sensitivity and 88.89% specificity. HEV Ag and HEV RNA have similar kinetics during the acute phase and self-limiting infection. In the FHF stage, HEV Ag and anti-HEV IgM have similar patterns of kinetics which could be the cause of liver damage. In conclusion, the HEV Ag assay can be used as a biomarker for predicting the consequences of HEV-1 infections which could be diagnostically useful for taking the appropriate measures to reduce the complications, especially for high-risk groups. Taylor & Francis 2021-05-18 /pmc/articles/PMC8143225/ /pubmed/34002677 http://dx.doi.org/10.1080/21505594.2021.1922027 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
El-Mokhtar, Mohamed A.
Karam-Allah Ramadan, Haidi
Abdel Hameed, Muhamad R.
M. Kamel, Ayat
A. Mandour, Sahar
Ali, Maha
Abdel-Malek, Mohamed A. Y.
M. Abd El-Kareem, Doaa
Adel, Sara
H. Salama, Eman
Khalaf, Khaled Abo Bakr
Sayed, Ibrahim M.
Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection
title Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection
title_full Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection
title_fullStr Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection
title_full_unstemmed Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection
title_short Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection
title_sort evaluation of hepatitis e antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis e virus genotype 1 infection
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143225/
https://www.ncbi.nlm.nih.gov/pubmed/34002677
http://dx.doi.org/10.1080/21505594.2021.1922027
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