Efficient Bioflocculation of Chlorella vulgaris with a Chitosan and Walnut Protein Extract

SIMPLE SUMMARY: With the increase in population size, global climate changes, and the improvement of living standards, the fossil fuel resources may run out in the future. Microalgae have been considered the next generation of sustainable and renewable feedstock to produce biofuel and a large spectrum of high-value products, such as healthy oils, carotenoids, and proteins. Unlike terrestrial plants, the production of added-value chemicals from microalgal species is not seasonal; they can be grown under climate-independent conditions in bioreactors; can use wastewater as a source of nutrients, contributing to wastewater treatment; and can convert CO(2) into organic compounds more efficiently. However, the utilization of microalgal biomass is heavily dependent on microalgal biomass harvesting and concentration technology. Flocculation represents a relatively low-cost and efficient approach for the harvesting of microalgal biomass at a large scale. However, in traditional flocculation, most of the chemical flocculants covalently bind to the microalgal surfaces, contaminating the final product, which significantly limits their application. This study aims to develop an efficient and convenient bioflocculation technique to harvest microalgae. ABSTRACT: Bioflocculation represents an attractive technology for harvesting microalgae with the potential additive effect of flocculants on the production of added-value chemicals. Chitosan, as a cationic polyelectrolyte, is widely used as a non-toxic, biodegradable bioflocculant for many algal species. The high cost of chitosan makes its large-scale application economically challenging, which triggered research on reducing its amount using co-flocculation with other components. In our study, chitosan alone at a concentration 10 mg/L showed up to an 89% flocculation efficiency for Chlorella vulgaris. Walnut protein extract (WPE) alone showed a modest level (up to 40%) of flocculation efficiency. The presence of WPE increased chitosan’s flocculation efficiency up to 98% at a reduced concentration of chitosan (6 mg/L). Assessment of co-flocculation efficiency at a broad region of pH showed the maximum harvesting efficiency at a neutral pH. Fourier transform infrared spectroscopy, floc size analysis, and microscopy suggested that the dual flocculation with chitosan and walnut protein is a result of the chemical interaction between the components that form a web-like structure, enhancing the bridging and sweeping ability of chitosan. Co-flocculation of chitosan with walnut protein extract, a low-value leftover from walnut oil production, represents an efficient and relatively cheap system for microalgal harvesting.