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Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS

A simple and fast analytical method able to simultaneously identify and quantify 17 endogenous and exogenous steroidal hormones was developed in bovine and equine blood using UHPLC-MS/MS. A total amount of 500 µL of sample was deproteinized with 500 µL of a mixture of methanol and zinc sulfate and e...

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Autores principales: Caprioli, Giovanni, Genangeli, Michele, Mustafa, Ahmed M., Petrelli, Riccardo, Ricciutelli, Massimo, Sagratini, Gianni, Sartori, Stefano, Laus, Fulvio, Vittori, Sauro, Cortese, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143330/
https://www.ncbi.nlm.nih.gov/pubmed/33919404
http://dx.doi.org/10.3390/ph14050393
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author Caprioli, Giovanni
Genangeli, Michele
Mustafa, Ahmed M.
Petrelli, Riccardo
Ricciutelli, Massimo
Sagratini, Gianni
Sartori, Stefano
Laus, Fulvio
Vittori, Sauro
Cortese, Manuela
author_facet Caprioli, Giovanni
Genangeli, Michele
Mustafa, Ahmed M.
Petrelli, Riccardo
Ricciutelli, Massimo
Sagratini, Gianni
Sartori, Stefano
Laus, Fulvio
Vittori, Sauro
Cortese, Manuela
author_sort Caprioli, Giovanni
collection PubMed
description A simple and fast analytical method able to simultaneously identify and quantify 17 endogenous and exogenous steroidal hormones was developed in bovine and equine blood using UHPLC-MS/MS. A total amount of 500 µL of sample was deproteinized with 500 µL of a mixture of methanol and zinc sulfate and evaporated. The mixture was reconstituted with 50 µL of a solution of 25% methanol and injected in the UHPLC-MS/MS triple quadrupole. The correlation coefficients of the calibration curves of the analyzed compounds were in the range of 0.9932–0.9999, and the limits of detection and quantification were in the range of 0.023–1.833 and 0.069–5.5 ppb, respectively. The developed method showed a high sensitivity and qualitative aspects allowing the detection and quantification of all steroids in equine and bovine blood. Moreover, the detection limit of testosterone (50 ppt) is half of the threshold admitted in plasma (100 ppt). Once validated, the method was used to quantify 17 steroid hormones in both bovine and equine blood samples. The primary endogenous compounds detected were corticosterone (range 0.28–0.60 ppb) and cortisol (range 0.44–10.00 ppb), followed by androstenedione, testosterone and 11-deoxycortisol.
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spelling pubmed-81433302021-05-25 Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS Caprioli, Giovanni Genangeli, Michele Mustafa, Ahmed M. Petrelli, Riccardo Ricciutelli, Massimo Sagratini, Gianni Sartori, Stefano Laus, Fulvio Vittori, Sauro Cortese, Manuela Pharmaceuticals (Basel) Article A simple and fast analytical method able to simultaneously identify and quantify 17 endogenous and exogenous steroidal hormones was developed in bovine and equine blood using UHPLC-MS/MS. A total amount of 500 µL of sample was deproteinized with 500 µL of a mixture of methanol and zinc sulfate and evaporated. The mixture was reconstituted with 50 µL of a solution of 25% methanol and injected in the UHPLC-MS/MS triple quadrupole. The correlation coefficients of the calibration curves of the analyzed compounds were in the range of 0.9932–0.9999, and the limits of detection and quantification were in the range of 0.023–1.833 and 0.069–5.5 ppb, respectively. The developed method showed a high sensitivity and qualitative aspects allowing the detection and quantification of all steroids in equine and bovine blood. Moreover, the detection limit of testosterone (50 ppt) is half of the threshold admitted in plasma (100 ppt). Once validated, the method was used to quantify 17 steroid hormones in both bovine and equine blood samples. The primary endogenous compounds detected were corticosterone (range 0.28–0.60 ppb) and cortisol (range 0.44–10.00 ppb), followed by androstenedione, testosterone and 11-deoxycortisol. MDPI 2021-04-21 /pmc/articles/PMC8143330/ /pubmed/33919404 http://dx.doi.org/10.3390/ph14050393 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caprioli, Giovanni
Genangeli, Michele
Mustafa, Ahmed M.
Petrelli, Riccardo
Ricciutelli, Massimo
Sagratini, Gianni
Sartori, Stefano
Laus, Fulvio
Vittori, Sauro
Cortese, Manuela
Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
title Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
title_full Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
title_fullStr Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
title_full_unstemmed Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
title_short Quantification of 17 Endogenous and Exogenous Steroidal Hormones in Equine and Bovine Blood for Doping Control with UHPLC-MS/MS
title_sort quantification of 17 endogenous and exogenous steroidal hormones in equine and bovine blood for doping control with uhplc-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143330/
https://www.ncbi.nlm.nih.gov/pubmed/33919404
http://dx.doi.org/10.3390/ph14050393
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