Characterization of Bottlenose Dolphin (Tursiops truncatus) Sperm Based on Morphometric Traits

SIMPLE SUMMARY: Dolphins are one of the best adapted aquatic mammalians in captivity. While these animals can reproduce naturally in aquaria, many aspects related to their reproduction are still unknown. Their behavior, sociability with humans and ability to learn make dolphins ideal subjects for investigating their reproduction features for conservation programs aimed at this aquatic species and others. It is known that dolphins use a multi-male mating strategy, in which sperm competition could play a fundamental role. This study aims to characterize the shape and dimensions of dolphin sperm from two mature males and putatively classifies them into subpopulations. Moreover, the influence of sex hormone levels (testosterone) and refrigeration (temperature and storage period) with sperm dimension was evaluated. The results indicated that sperm dimensions and shape differed between the two males studied and that the sperm of both males could be classified into two subpopulations depending on their dimensions. Moreover, both testosterone levels and refrigeration were seen to influence sperm dimensions. This investigation provides new insights into sperm competition in dolphin species, and the results could be extrapolated to other endangered aquatic mammalian species. ABSTRACT: Bottlenose dolphin (Tursiops truncatus) males follow many reproductive strategies to ensure their paternity. However, little is known about the sperm traits, including morphometric features, that contribute to their reproductive success. Our aim was to study dolphin sperm morphometry (a total of 13 parameters) in two adult males to evaluate (i) presumptive sperm subpopulations, (ii) the correlation of sperm morphometry with testosterone levels and (iii) the effect of refrigerated storage on the sperm morphometry. Sperm populations were classified into four principal components (PCs) based on morphometry (>94% of cumulative variance). The PCs clustered into two different sperm subpopulations, which differed between males. Furthermore, the levels of serum testosterone were positively correlated with the length of the midpiece but negatively correlated with head width and the principal piece, flagellum and total sperm lengths. Most of the sperm morphometric parameters changed during the storage period (day 1 vs. day 7), but only the principal piece length was affected by the storage temperature (5 °C vs. 15 °C). This is the first study to identify dolphin sperm subpopulations based on morphometry and the influence of serum testosterone and refrigeration on sperm morphometry.