Worker Size Diversity Has No Effect on Overwintering Success under Natural Conditions in the Ant Temnothorax nylanderi

SIMPLE SUMMARY: Winter is a harsh season for organisms living in temperate zones. Winter is often associated with starvation and cold temperatures, and these pressures can strongly affect organism survival. Living in groups can help these animals to cope with winter pressures. Social groups contain individuals which can vary in different ways: physiology, behavior, morphology, etc. In social insects such as ants, worker size leads to different responses to starvation and cold temperature in the laboratory. In this study, we investigated whether worker size affects colony and individual survival under natural conditions. We manipulated both worker size diversity and mean worker size within colonies of the ant Temnothorax nylanderi, reintroduced them in the field, and measured colony survival after overwintering. We found similar colony and individual (both adults and young) survival during winter between treatment colonies with reduced size diversity and/or manipulated mean worker size compared to control colonies with unmanipulated worker size. This result highlights that worker size diversity has no influence on colony performance in this species and more broadly questions the interest of worker size in social insect species with moderate worker size diversity. We discuss the potential sources of worker size diversity, including social context and selfish behavior. ABSTRACT: Winter is a difficult period for animals that live in temperate zones. It can inflict high mortality or induce weight loss with potential consequences on performance during the growing season. Social groups include individuals of various ages and sizes. This diversity may improve the ability of groups to buffer winter disturbances such as starvation or cold temperature. Studies focusing on the buffering role of social traits such as mean size and diversity of group members under winter conditions are mainly performed in the laboratory and investigate the effect of starvation or cold separately. Here, we experimentally decreased worker size diversity and manipulated worker mean size within colonies in order to study the effect on overwintering survival in the ant Temnothorax nylanderi. Colonies were placed under natural conditions during winter. Colony survival was high during winter and similar in all treatments with no effect of worker size diversity and mean worker size. Higher brood survival was positively correlated with colony size (i.e., the number of workers). Our results show that the higher resistance of larger individuals against cold or starvation stresses observed in the laboratory does not directly translate into higher colony survival in the field. We discuss our results in the light of mechanisms that could explain the possible non-adaptive size diversity in social species.