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Identification of a blockade epitope of human norovirus GII.17
Human noroviruses are the dominant causative agent of acute viral gastroenteritis worldwide. During the winter of 2014–2015, genotype GII.17 cluster IIIb strains emerged as the leading cause of norovirus infection in Asia and later spread to other parts of the world. It is speculated that mutation a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143627/ https://www.ncbi.nlm.nih.gov/pubmed/33929932 http://dx.doi.org/10.1080/22221751.2021.1925162 |
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author | Yi, Yufang Wang, Xiaoli Wang, Shuxia Xiong, Pei Liu, Qingwei Zhang, Chao Yin, Feifei Huang, Zhong |
author_facet | Yi, Yufang Wang, Xiaoli Wang, Shuxia Xiong, Pei Liu, Qingwei Zhang, Chao Yin, Feifei Huang, Zhong |
author_sort | Yi, Yufang |
collection | PubMed |
description | Human noroviruses are the dominant causative agent of acute viral gastroenteritis worldwide. During the winter of 2014–2015, genotype GII.17 cluster IIIb strains emerged as the leading cause of norovirus infection in Asia and later spread to other parts of the world. It is speculated that mutation at blockade epitopes may have resulted in virus escape from herd immunity, leading to the emergence of GII.17 cluster IIIb variants. Here, we identify a GII.17 cluster IIIb-specific blockade epitope by monoclonal antibody (mAb)-based epitope mapping. Four mAbs (designated as M1 to M4) were generated from mice immunized with virus-like particle (VLP) of a GII.17 cluster IIIb strain. Among them, M1 and M3 reacted specifically with the cluster IIIb VLP but not with the VLPs from clusters II or IIIa. Moreover, M1 and M3 dose-dependently blocked cluster IIIb VLP binding with its ligand, histo-blood group antigens (HBGAs). Epitope mapping revealed that M1 and M3 recognized the same highly exposed epitope consisting of residues 293–296 and 299 in the capsid protein VP1. Sequence alignment showed that the M1/M3 epitope sequence is highly variable among different GII.17 clusters whereas it is identical for cluster IIIIb strains. These data define a dominant blockade epitope of GII.17 norovirus and provide evidence that blockade epitope evolution contributes to the emergence of GII.17 cluster IIIb strains. |
format | Online Article Text |
id | pubmed-8143627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-81436272021-06-07 Identification of a blockade epitope of human norovirus GII.17 Yi, Yufang Wang, Xiaoli Wang, Shuxia Xiong, Pei Liu, Qingwei Zhang, Chao Yin, Feifei Huang, Zhong Emerg Microbes Infect Research Article Human noroviruses are the dominant causative agent of acute viral gastroenteritis worldwide. During the winter of 2014–2015, genotype GII.17 cluster IIIb strains emerged as the leading cause of norovirus infection in Asia and later spread to other parts of the world. It is speculated that mutation at blockade epitopes may have resulted in virus escape from herd immunity, leading to the emergence of GII.17 cluster IIIb variants. Here, we identify a GII.17 cluster IIIb-specific blockade epitope by monoclonal antibody (mAb)-based epitope mapping. Four mAbs (designated as M1 to M4) were generated from mice immunized with virus-like particle (VLP) of a GII.17 cluster IIIb strain. Among them, M1 and M3 reacted specifically with the cluster IIIb VLP but not with the VLPs from clusters II or IIIa. Moreover, M1 and M3 dose-dependently blocked cluster IIIb VLP binding with its ligand, histo-blood group antigens (HBGAs). Epitope mapping revealed that M1 and M3 recognized the same highly exposed epitope consisting of residues 293–296 and 299 in the capsid protein VP1. Sequence alignment showed that the M1/M3 epitope sequence is highly variable among different GII.17 clusters whereas it is identical for cluster IIIIb strains. These data define a dominant blockade epitope of GII.17 norovirus and provide evidence that blockade epitope evolution contributes to the emergence of GII.17 cluster IIIb strains. Taylor & Francis 2021-05-18 /pmc/articles/PMC8143627/ /pubmed/33929932 http://dx.doi.org/10.1080/22221751.2021.1925162 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yi, Yufang Wang, Xiaoli Wang, Shuxia Xiong, Pei Liu, Qingwei Zhang, Chao Yin, Feifei Huang, Zhong Identification of a blockade epitope of human norovirus GII.17 |
title | Identification of a blockade epitope of human norovirus GII.17 |
title_full | Identification of a blockade epitope of human norovirus GII.17 |
title_fullStr | Identification of a blockade epitope of human norovirus GII.17 |
title_full_unstemmed | Identification of a blockade epitope of human norovirus GII.17 |
title_short | Identification of a blockade epitope of human norovirus GII.17 |
title_sort | identification of a blockade epitope of human norovirus gii.17 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143627/ https://www.ncbi.nlm.nih.gov/pubmed/33929932 http://dx.doi.org/10.1080/22221751.2021.1925162 |
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