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Design principles of the ESCRT-III Vps24-Vps2 module

ESCRT-III polymerization is required for all endosomal sorting complex required for transport (ESCRT)-dependent events in the cell. However, the relative contributions of the eight ESCRT-III subunits differ between each process. The minimal features of ESCRT-III proteins necessary for function and t...

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Autores principales: Banjade, Sudeep, Shah, Yousuf H, Tang, Shaogeng, Emr, Scott D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143795/
https://www.ncbi.nlm.nih.gov/pubmed/34028356
http://dx.doi.org/10.7554/eLife.67709
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author Banjade, Sudeep
Shah, Yousuf H
Tang, Shaogeng
Emr, Scott D
author_facet Banjade, Sudeep
Shah, Yousuf H
Tang, Shaogeng
Emr, Scott D
author_sort Banjade, Sudeep
collection PubMed
description ESCRT-III polymerization is required for all endosomal sorting complex required for transport (ESCRT)-dependent events in the cell. However, the relative contributions of the eight ESCRT-III subunits differ between each process. The minimal features of ESCRT-III proteins necessary for function and the role for the multiple ESCRT-III subunits remain unclear. To identify essential features of ESCRT-III subunits, we previously studied the polymerization mechanisms of two ESCRT-III subunits Snf7 and Vps24, identifying the association of the helix-4 region of Snf7 with the helix-1 region of Vps24 (Banjade et al., 2019a). Here, we find that mutations in the helix-1 region of another ESCRT-III subunit Vps2 can functionally replace Vps24 in Saccharomyces cerevisiae. Engineering and genetic selections revealed the required features of both subunits. Our data allow us to propose three minimal features required for ESCRT-III function – spiral formation, lateral association of the spirals through heteropolymerization, and binding to the AAA + ATPase Vps4 for dynamic remodeling.
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spelling pubmed-81437952021-05-26 Design principles of the ESCRT-III Vps24-Vps2 module Banjade, Sudeep Shah, Yousuf H Tang, Shaogeng Emr, Scott D eLife Biochemistry and Chemical Biology ESCRT-III polymerization is required for all endosomal sorting complex required for transport (ESCRT)-dependent events in the cell. However, the relative contributions of the eight ESCRT-III subunits differ between each process. The minimal features of ESCRT-III proteins necessary for function and the role for the multiple ESCRT-III subunits remain unclear. To identify essential features of ESCRT-III subunits, we previously studied the polymerization mechanisms of two ESCRT-III subunits Snf7 and Vps24, identifying the association of the helix-4 region of Snf7 with the helix-1 region of Vps24 (Banjade et al., 2019a). Here, we find that mutations in the helix-1 region of another ESCRT-III subunit Vps2 can functionally replace Vps24 in Saccharomyces cerevisiae. Engineering and genetic selections revealed the required features of both subunits. Our data allow us to propose three minimal features required for ESCRT-III function – spiral formation, lateral association of the spirals through heteropolymerization, and binding to the AAA + ATPase Vps4 for dynamic remodeling. eLife Sciences Publications, Ltd 2021-05-24 /pmc/articles/PMC8143795/ /pubmed/34028356 http://dx.doi.org/10.7554/eLife.67709 Text en © 2021, Banjade et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Banjade, Sudeep
Shah, Yousuf H
Tang, Shaogeng
Emr, Scott D
Design principles of the ESCRT-III Vps24-Vps2 module
title Design principles of the ESCRT-III Vps24-Vps2 module
title_full Design principles of the ESCRT-III Vps24-Vps2 module
title_fullStr Design principles of the ESCRT-III Vps24-Vps2 module
title_full_unstemmed Design principles of the ESCRT-III Vps24-Vps2 module
title_short Design principles of the ESCRT-III Vps24-Vps2 module
title_sort design principles of the escrt-iii vps24-vps2 module
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143795/
https://www.ncbi.nlm.nih.gov/pubmed/34028356
http://dx.doi.org/10.7554/eLife.67709
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