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Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo
Developing strategies that promote the resolution of vascular inflammation and atherosclerosis remains a major therapeutic challenge. Here, we show that exosomes produced by naive bone marrow-derived macrophages (BMDM-exo) contain anti-inflammatory microRNA-99a/146b/378a that are further increased i...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143919/ https://www.ncbi.nlm.nih.gov/pubmed/32668250 http://dx.doi.org/10.1016/j.celrep.2020.107881 |
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author | Bouchareychas, Laura Duong, Phat Covarrubias, Sergio Alsop, Eric Phu, Tuan Anh Chung, Allen Gomes, Michael Wong, David Meechoovet, Bessie Capili, Allyson Yamamoto, Ryo Nakauchi, Hiromitsu McManus, Michael T. Carpenter, Susan Van Keuren-Jensen, Kendall Raffai, Robert L. |
author_facet | Bouchareychas, Laura Duong, Phat Covarrubias, Sergio Alsop, Eric Phu, Tuan Anh Chung, Allen Gomes, Michael Wong, David Meechoovet, Bessie Capili, Allyson Yamamoto, Ryo Nakauchi, Hiromitsu McManus, Michael T. Carpenter, Susan Van Keuren-Jensen, Kendall Raffai, Robert L. |
author_sort | Bouchareychas, Laura |
collection | PubMed |
description | Developing strategies that promote the resolution of vascular inflammation and atherosclerosis remains a major therapeutic challenge. Here, we show that exosomes produced by naive bone marrow-derived macrophages (BMDM-exo) contain anti-inflammatory microRNA-99a/146b/378a that are further increased in exosomes produced by BMDM polarized with IL-4 (BMDM-IL-4-exo). These exosomal microRNAs suppress inflammation by targeting NF-κB and TNF-α signaling and foster M2 polarization in recipient macrophages. Repeated infusions of BMDM-IL-4-exo into Apoe(−/−) mice fed a Western diet reduce excessive hematopoiesis in the bone marrow and thereby the number of myeloid cells in the circulation and macrophages in aortic root lesions. This also leads to a reduction in necrotic lesion areas that collectively stabilize atheroma. Thus, BMDM-IL-4-exo may represent a useful therapeutic approach for atherosclerosis and other inflammatory disorders by targeting NF-κB and TNF-α via microRNA cargo delivery. |
format | Online Article Text |
id | pubmed-8143919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-81439192021-05-24 Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo Bouchareychas, Laura Duong, Phat Covarrubias, Sergio Alsop, Eric Phu, Tuan Anh Chung, Allen Gomes, Michael Wong, David Meechoovet, Bessie Capili, Allyson Yamamoto, Ryo Nakauchi, Hiromitsu McManus, Michael T. Carpenter, Susan Van Keuren-Jensen, Kendall Raffai, Robert L. Cell Rep Article Developing strategies that promote the resolution of vascular inflammation and atherosclerosis remains a major therapeutic challenge. Here, we show that exosomes produced by naive bone marrow-derived macrophages (BMDM-exo) contain anti-inflammatory microRNA-99a/146b/378a that are further increased in exosomes produced by BMDM polarized with IL-4 (BMDM-IL-4-exo). These exosomal microRNAs suppress inflammation by targeting NF-κB and TNF-α signaling and foster M2 polarization in recipient macrophages. Repeated infusions of BMDM-IL-4-exo into Apoe(−/−) mice fed a Western diet reduce excessive hematopoiesis in the bone marrow and thereby the number of myeloid cells in the circulation and macrophages in aortic root lesions. This also leads to a reduction in necrotic lesion areas that collectively stabilize atheroma. Thus, BMDM-IL-4-exo may represent a useful therapeutic approach for atherosclerosis and other inflammatory disorders by targeting NF-κB and TNF-α via microRNA cargo delivery. 2020-07-14 /pmc/articles/PMC8143919/ /pubmed/32668250 http://dx.doi.org/10.1016/j.celrep.2020.107881 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Bouchareychas, Laura Duong, Phat Covarrubias, Sergio Alsop, Eric Phu, Tuan Anh Chung, Allen Gomes, Michael Wong, David Meechoovet, Bessie Capili, Allyson Yamamoto, Ryo Nakauchi, Hiromitsu McManus, Michael T. Carpenter, Susan Van Keuren-Jensen, Kendall Raffai, Robert L. Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo |
title | Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo |
title_full | Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo |
title_fullStr | Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo |
title_full_unstemmed | Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo |
title_short | Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo |
title_sort | macrophage exosomes resolve atherosclerosis by regulating hematopoiesis and inflammation via microrna cargo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143919/ https://www.ncbi.nlm.nih.gov/pubmed/32668250 http://dx.doi.org/10.1016/j.celrep.2020.107881 |
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