Cargando…

The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae

The number and classification of tribes in the leafhopper subfamily Typhlocybinae are not yet fully clear, and molecular data has recently been used to help resolve the problem. In this study, the mitochondrial genomes of Mitjaevia shibingensis Chen, Song & Webb, 2020 and M. dworakowskae Chen, S...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Xiaoxiao, Li, Can, Song, Yuehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144164/
https://www.ncbi.nlm.nih.gov/pubmed/34054318
http://dx.doi.org/10.3897/zookeys.1037.63671
_version_ 1783696904900575232
author Chen, Xiaoxiao
Li, Can
Song, Yuehua
author_facet Chen, Xiaoxiao
Li, Can
Song, Yuehua
author_sort Chen, Xiaoxiao
collection PubMed
description The number and classification of tribes in the leafhopper subfamily Typhlocybinae are not yet fully clear, and molecular data has recently been used to help resolve the problem. In this study, the mitochondrial genomes of Mitjaevia shibingensis Chen, Song & Webb, 2020 and M. dworakowskae Chen, Song & Webb, 2020 of the tribe Erythroneurini (Cicadellidae, Typhlocybinae) were sequenced. Most protein-coding genes (PCGs) start with ATN and end with TAA or TAG, and the AT content of these three codons were found differ from previous results that show that the first codon has the highest incidence. Two rRNA genes are highly conserved, and the AT content in 16S is higher than that of 12S. The nucleotide diversity and genetic distance among 13 PCGs of the four tribes from Typhlocybinae show that Empoascini nucleotide diversity is significantly less than in the other three tribes, and have the largest distance from the others, while Typhlocybini and Zyginellini have the smallest distance, indicating that the relationship between the two is the closest. The nad2, nad4, nad4L, and nad5 genes have greater nucleotide diversity, showing potential for use as the main markers for species identification. The phylogenetic analysis yielded a well-supported topology with most branches receiving maximum support and a few branches pertaining to relationships within Zyginellini and Typhlocybini receiving lower support. The species of these two tribes are intertwined, and it was impossible to resolve them into separate branches. In addition, the tribes Empoascini and Erythroneurini were recovered as monophyletic, and Alebrini was placed at the base of the tree as the most primitive. These results are broadly in line with other molecular phylogenetical studies which differ from traditional morphological classification.
format Online
Article
Text
id pubmed-8144164
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Pensoft Publishers
record_format MEDLINE/PubMed
spelling pubmed-81441642021-05-27 The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae Chen, Xiaoxiao Li, Can Song, Yuehua Zookeys Research Article The number and classification of tribes in the leafhopper subfamily Typhlocybinae are not yet fully clear, and molecular data has recently been used to help resolve the problem. In this study, the mitochondrial genomes of Mitjaevia shibingensis Chen, Song & Webb, 2020 and M. dworakowskae Chen, Song & Webb, 2020 of the tribe Erythroneurini (Cicadellidae, Typhlocybinae) were sequenced. Most protein-coding genes (PCGs) start with ATN and end with TAA or TAG, and the AT content of these three codons were found differ from previous results that show that the first codon has the highest incidence. Two rRNA genes are highly conserved, and the AT content in 16S is higher than that of 12S. The nucleotide diversity and genetic distance among 13 PCGs of the four tribes from Typhlocybinae show that Empoascini nucleotide diversity is significantly less than in the other three tribes, and have the largest distance from the others, while Typhlocybini and Zyginellini have the smallest distance, indicating that the relationship between the two is the closest. The nad2, nad4, nad4L, and nad5 genes have greater nucleotide diversity, showing potential for use as the main markers for species identification. The phylogenetic analysis yielded a well-supported topology with most branches receiving maximum support and a few branches pertaining to relationships within Zyginellini and Typhlocybini receiving lower support. The species of these two tribes are intertwined, and it was impossible to resolve them into separate branches. In addition, the tribes Empoascini and Erythroneurini were recovered as monophyletic, and Alebrini was placed at the base of the tree as the most primitive. These results are broadly in line with other molecular phylogenetical studies which differ from traditional morphological classification. Pensoft Publishers 2021-05-17 /pmc/articles/PMC8144164/ /pubmed/34054318 http://dx.doi.org/10.3897/zookeys.1037.63671 Text en Xiaoxiao Chen, Can Li, Yuehua Song https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Xiaoxiao
Li, Can
Song, Yuehua
The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae
title The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae
title_full The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae
title_fullStr The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae
title_full_unstemmed The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae
title_short The complete mitochondrial genomes of two erythroneurine leafhoppers (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) with assessment of the phylogenetic status and relationships of tribes of Typhlocybinae
title_sort complete mitochondrial genomes of two erythroneurine leafhoppers (hemiptera, cicadellidae, typhlocybinae, erythroneurini) with assessment of the phylogenetic status and relationships of tribes of typhlocybinae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144164/
https://www.ncbi.nlm.nih.gov/pubmed/34054318
http://dx.doi.org/10.3897/zookeys.1037.63671
work_keys_str_mv AT chenxiaoxiao thecompletemitochondrialgenomesoftwoerythroneurineleafhoppershemipteracicadellidaetyphlocybinaeerythroneuriniwithassessmentofthephylogeneticstatusandrelationshipsoftribesoftyphlocybinae
AT lican thecompletemitochondrialgenomesoftwoerythroneurineleafhoppershemipteracicadellidaetyphlocybinaeerythroneuriniwithassessmentofthephylogeneticstatusandrelationshipsoftribesoftyphlocybinae
AT songyuehua thecompletemitochondrialgenomesoftwoerythroneurineleafhoppershemipteracicadellidaetyphlocybinaeerythroneuriniwithassessmentofthephylogeneticstatusandrelationshipsoftribesoftyphlocybinae
AT chenxiaoxiao completemitochondrialgenomesoftwoerythroneurineleafhoppershemipteracicadellidaetyphlocybinaeerythroneuriniwithassessmentofthephylogeneticstatusandrelationshipsoftribesoftyphlocybinae
AT lican completemitochondrialgenomesoftwoerythroneurineleafhoppershemipteracicadellidaetyphlocybinaeerythroneuriniwithassessmentofthephylogeneticstatusandrelationshipsoftribesoftyphlocybinae
AT songyuehua completemitochondrialgenomesoftwoerythroneurineleafhoppershemipteracicadellidaetyphlocybinaeerythroneuriniwithassessmentofthephylogeneticstatusandrelationshipsoftribesoftyphlocybinae