Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation

Leaf trichomes play vital roles in plant resistance and the quality of tea. Basic helix-loop-helix (bHLH) transcription factors (TFs) play an important role in regulating plant development and growth. In this study, a total of 134 CsbHLH proteins were identified in the Camellia sinensis var. sinensi...

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Autores principales: Liu, Renjian, Wang, Yuyuan, Tang, Song, Cai, Jiarong, Liu, Shaoqun, Zheng, Peng, Sun, Binmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144589/
https://www.ncbi.nlm.nih.gov/pubmed/34031482
http://dx.doi.org/10.1038/s41598-021-90205-7
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author Liu, Renjian
Wang, Yuyuan
Tang, Song
Cai, Jiarong
Liu, Shaoqun
Zheng, Peng
Sun, Binmei
author_facet Liu, Renjian
Wang, Yuyuan
Tang, Song
Cai, Jiarong
Liu, Shaoqun
Zheng, Peng
Sun, Binmei
author_sort Liu, Renjian
collection PubMed
description Leaf trichomes play vital roles in plant resistance and the quality of tea. Basic helix-loop-helix (bHLH) transcription factors (TFs) play an important role in regulating plant development and growth. In this study, a total of 134 CsbHLH proteins were identified in the Camellia sinensis var. sinensis (CSS) genome. They were divided into 17 subgroups according to the Arabidopsis thaliana classification. Phylogenetic tree analysis indicated that members of subgroups IIIc-I and IIIc-II might be associated with trichome formation. The expression patterns of CsbHLH116, CsbHLH133, CsbHLH060, CsbHLH028, CsbHLH024, CsbHLH112 and CsbHLH053 from clusters 1, 3 and 5 were similar to the trichome distribution in tea plants. CsbHLH024 and CsbHLH133 were located in the cell nucleus and possessed transcriptional activation ability. They could interact with CsTTG1, which is a regulator of tea trichome formation. This study provides useful information for further research on the function of CsbHLHs in trichome formation.
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spelling pubmed-81445892021-05-26 Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation Liu, Renjian Wang, Yuyuan Tang, Song Cai, Jiarong Liu, Shaoqun Zheng, Peng Sun, Binmei Sci Rep Article Leaf trichomes play vital roles in plant resistance and the quality of tea. Basic helix-loop-helix (bHLH) transcription factors (TFs) play an important role in regulating plant development and growth. In this study, a total of 134 CsbHLH proteins were identified in the Camellia sinensis var. sinensis (CSS) genome. They were divided into 17 subgroups according to the Arabidopsis thaliana classification. Phylogenetic tree analysis indicated that members of subgroups IIIc-I and IIIc-II might be associated with trichome formation. The expression patterns of CsbHLH116, CsbHLH133, CsbHLH060, CsbHLH028, CsbHLH024, CsbHLH112 and CsbHLH053 from clusters 1, 3 and 5 were similar to the trichome distribution in tea plants. CsbHLH024 and CsbHLH133 were located in the cell nucleus and possessed transcriptional activation ability. They could interact with CsTTG1, which is a regulator of tea trichome formation. This study provides useful information for further research on the function of CsbHLHs in trichome formation. Nature Publishing Group UK 2021-05-24 /pmc/articles/PMC8144589/ /pubmed/34031482 http://dx.doi.org/10.1038/s41598-021-90205-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liu, Renjian
Wang, Yuyuan
Tang, Song
Cai, Jiarong
Liu, Shaoqun
Zheng, Peng
Sun, Binmei
Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation
title Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation
title_full Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation
title_fullStr Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation
title_full_unstemmed Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation
title_short Genome-wide identification of the tea plant bHLH transcription factor family and discovery of candidate regulators of trichome formation
title_sort genome-wide identification of the tea plant bhlh transcription factor family and discovery of candidate regulators of trichome formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144589/
https://www.ncbi.nlm.nih.gov/pubmed/34031482
http://dx.doi.org/10.1038/s41598-021-90205-7
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