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The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer
We aimed to identify and investigate genes that are essential for the development of clear cell renal cell carcinoma (ccRCC) and sought to shed light on the mechanisms of its progression and create prognostic markers for the disease. We used real-time PCR to study the expression of 20 genes that wer...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144703/ https://www.ncbi.nlm.nih.gov/pubmed/34046336 http://dx.doi.org/10.3389/fonc.2021.615787 |
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author | Apanovich, Natalya Apanovich, Pavel Mansorunov, Danzan Kuzevanova, Anna Matveev, Vsevolod Karpukhin, Alexander |
author_facet | Apanovich, Natalya Apanovich, Pavel Mansorunov, Danzan Kuzevanova, Anna Matveev, Vsevolod Karpukhin, Alexander |
author_sort | Apanovich, Natalya |
collection | PubMed |
description | We aimed to identify and investigate genes that are essential for the development of clear cell renal cell carcinoma (ccRCC) and sought to shed light on the mechanisms of its progression and create prognostic markers for the disease. We used real-time PCR to study the expression of 20 genes that were preliminarily selected based on their differential expression in ccRCC, in 68 paired tumor/normal samples. Upon ccRCC progression, seven genes that showed an initial increase in expression showed decreased expression. The genes whose expression levels did not significantly change during progression were associated mainly with metabolic and inflammatory processes. The first group included CA9, NDUFA4L2, EGLN3, BHLHE41, VWF, IGFBP3, and ANGPTL4, whose expression levels were coordinately decreased during tumor progression. This expression coordination and gene function is related to the needs of tumor development at different stages. Specifically, the high correlation coefficient of EGLN3 and NDUFA4L2 expression may indicate the importance of the coordinated regulation of glycolysis and mitochondrial metabolism. A panel of CA9, EGLN3, BHLHE41, and VWF enabled the prediction of survival for more than 3.5 years in patients with ccRCC, with a probability close to 90%. Therefore, a coordinated change in the expression of a gene group during ccRCC progression was detected, and a new panel of markers for individual survival prognosis was identified. |
format | Online Article Text |
id | pubmed-8144703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81447032021-05-26 The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer Apanovich, Natalya Apanovich, Pavel Mansorunov, Danzan Kuzevanova, Anna Matveev, Vsevolod Karpukhin, Alexander Front Oncol Oncology We aimed to identify and investigate genes that are essential for the development of clear cell renal cell carcinoma (ccRCC) and sought to shed light on the mechanisms of its progression and create prognostic markers for the disease. We used real-time PCR to study the expression of 20 genes that were preliminarily selected based on their differential expression in ccRCC, in 68 paired tumor/normal samples. Upon ccRCC progression, seven genes that showed an initial increase in expression showed decreased expression. The genes whose expression levels did not significantly change during progression were associated mainly with metabolic and inflammatory processes. The first group included CA9, NDUFA4L2, EGLN3, BHLHE41, VWF, IGFBP3, and ANGPTL4, whose expression levels were coordinately decreased during tumor progression. This expression coordination and gene function is related to the needs of tumor development at different stages. Specifically, the high correlation coefficient of EGLN3 and NDUFA4L2 expression may indicate the importance of the coordinated regulation of glycolysis and mitochondrial metabolism. A panel of CA9, EGLN3, BHLHE41, and VWF enabled the prediction of survival for more than 3.5 years in patients with ccRCC, with a probability close to 90%. Therefore, a coordinated change in the expression of a gene group during ccRCC progression was detected, and a new panel of markers for individual survival prognosis was identified. Frontiers Media S.A. 2021-05-11 /pmc/articles/PMC8144703/ /pubmed/34046336 http://dx.doi.org/10.3389/fonc.2021.615787 Text en Copyright © 2021 Apanovich, Apanovich, Mansorunov, Kuzevanova, Matveev and Karpukhin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Apanovich, Natalya Apanovich, Pavel Mansorunov, Danzan Kuzevanova, Anna Matveev, Vsevolod Karpukhin, Alexander The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer |
title | The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer |
title_full | The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer |
title_fullStr | The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer |
title_full_unstemmed | The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer |
title_short | The Choice of Candidates in Survival Markers Based on Coordinated Gene Expression in Renal Cancer |
title_sort | choice of candidates in survival markers based on coordinated gene expression in renal cancer |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144703/ https://www.ncbi.nlm.nih.gov/pubmed/34046336 http://dx.doi.org/10.3389/fonc.2021.615787 |
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