Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system

Environmental stimuli evoke transient increases of the cytosolic Ca(2+) level. To identify upstream components of Ca(2+) signaling, we have optimized two forward genetic screening systems based on Ca(2+) reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS...

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Detalles Bibliográficos
Autores principales: Sun, Shujing, Zhang, Xiaoyan, Chen, Kong, Zhu, Xiaohong, Zhao, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144734/
https://www.ncbi.nlm.nih.gov/pubmed/34041505
http://dx.doi.org/10.1016/j.xpro.2021.100558
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author Sun, Shujing
Zhang, Xiaoyan
Chen, Kong
Zhu, Xiaohong
Zhao, Yang
author_facet Sun, Shujing
Zhang, Xiaoyan
Chen, Kong
Zhu, Xiaohong
Zhao, Yang
author_sort Sun, Shujing
collection PubMed
description Environmental stimuli evoke transient increases of the cytosolic Ca(2+) level. To identify upstream components of Ca(2+) signaling, we have optimized two forward genetic screening systems based on Ca(2+) reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQsig6 EMS-mutagenized library was preferably used to screen the mutants with reduced Ca(2+) signal response due to its high effectiveness, while the AEQub EMS-mutagenized library was used for screening of the mutants with altered Ca(2+) signal response. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013).
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spelling pubmed-81447342021-05-25 Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system Sun, Shujing Zhang, Xiaoyan Chen, Kong Zhu, Xiaohong Zhao, Yang STAR Protoc Protocol Environmental stimuli evoke transient increases of the cytosolic Ca(2+) level. To identify upstream components of Ca(2+) signaling, we have optimized two forward genetic screening systems based on Ca(2+) reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQsig6 EMS-mutagenized library was preferably used to screen the mutants with reduced Ca(2+) signal response due to its high effectiveness, while the AEQub EMS-mutagenized library was used for screening of the mutants with altered Ca(2+) signal response. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013). Elsevier 2021-05-23 /pmc/articles/PMC8144734/ /pubmed/34041505 http://dx.doi.org/10.1016/j.xpro.2021.100558 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Sun, Shujing
Zhang, Xiaoyan
Chen, Kong
Zhu, Xiaohong
Zhao, Yang
Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system
title Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system
title_full Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system
title_fullStr Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system
title_full_unstemmed Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system
title_short Screening for Arabidopsis mutants with altered Ca(2+) signal response using aequorin-based Ca(2+) reporter system
title_sort screening for arabidopsis mutants with altered ca(2+) signal response using aequorin-based ca(2+) reporter system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144734/
https://www.ncbi.nlm.nih.gov/pubmed/34041505
http://dx.doi.org/10.1016/j.xpro.2021.100558
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