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Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells

Objective: To demonstrate the levels of parathyroid hormone secretion and genetic expressions of parathyroid hormone (PTH) and PTH1 receptor (PTH1R) genes in the dental pulp stem cells (DPSCs) from different age groups before and after induction of osteogenic differentiation. In addition, we also wa...

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Autores principales: Bhandi, Shilpa, Alkahtani, Ahmed, Reda, Rodolfo, Mashyakhy, Mohammed, Boreak, Nezar, Maganur, Prabhadevi C., Vishwanathaiah, Satish, Mehta, Deepak, Vyas, Nishant, Patil, Vikrant, Raj, A. Thirumal, Testarelli, Luca, Patil, Shankargouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144966/
https://www.ncbi.nlm.nih.gov/pubmed/33925324
http://dx.doi.org/10.3390/jpm11050349
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author Bhandi, Shilpa
Alkahtani, Ahmed
Reda, Rodolfo
Mashyakhy, Mohammed
Boreak, Nezar
Maganur, Prabhadevi C.
Vishwanathaiah, Satish
Mehta, Deepak
Vyas, Nishant
Patil, Vikrant
Raj, A. Thirumal
Testarelli, Luca
Patil, Shankargouda
author_facet Bhandi, Shilpa
Alkahtani, Ahmed
Reda, Rodolfo
Mashyakhy, Mohammed
Boreak, Nezar
Maganur, Prabhadevi C.
Vishwanathaiah, Satish
Mehta, Deepak
Vyas, Nishant
Patil, Vikrant
Raj, A. Thirumal
Testarelli, Luca
Patil, Shankargouda
author_sort Bhandi, Shilpa
collection PubMed
description Objective: To demonstrate the levels of parathyroid hormone secretion and genetic expressions of parathyroid hormone (PTH) and PTH1 receptor (PTH1R) genes in the dental pulp stem cells (DPSCs) from different age groups before and after induction of osteogenic differentiation. In addition, we also wanted to check their correlation with the degree of osteogenic differentiation. Methods: Human primary DPSCs from three age groups (milk tooth (SHEDs), 7–12 years old; young DPSCs (yDPSCs), 20–40 years old; old DPSCs (oDPSCs), 60+ years old) were characterized for mesenchymal stem cell (MSC) markers. DPSCs were subjected to osteogenic differentiation and functional staining. Gene expression levels were analyzed by qRT-PCR. Surface receptor analysis was done by flow cytometry. Comparative protein levels were evaluated by ELISA. Results: All SHEDs, yDPSCs, and oDPSCs were found to be expressing mesenchymal stem cell markers. SHEDs showed more mineralization than yDPSCs and oDPSCs after osteogenic induction. SHEDs exhibited higher expression of PTH and PTH1R before and after osteogenic induction, and after osteogenic induction, SHEDs showed more expression for RUNX2, ALPL, and OCN. Higher levels of PTH were observed in SHEDs and yDPSCs, and the number of PTH1R positive cells was relatively lower in yDPSCs and oDPSCs than in SHEDs. After osteogenic induction, SHEDs were superior in the secretion of OPG, and the secretions of ALPL and PTH and the number of PTH1R positive cells were relatively low in the oDPSCs. Conclusions: The therapeutic quality of dental pulp stem cells is largely based on their ability to retain their stemness characteristics. This study emphasizes the criterion of aging, which affects the secretion of PTH by these cells, which in turn attenuates their osteogenic potential.
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spelling pubmed-81449662021-05-26 Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells Bhandi, Shilpa Alkahtani, Ahmed Reda, Rodolfo Mashyakhy, Mohammed Boreak, Nezar Maganur, Prabhadevi C. Vishwanathaiah, Satish Mehta, Deepak Vyas, Nishant Patil, Vikrant Raj, A. Thirumal Testarelli, Luca Patil, Shankargouda J Pers Med Article Objective: To demonstrate the levels of parathyroid hormone secretion and genetic expressions of parathyroid hormone (PTH) and PTH1 receptor (PTH1R) genes in the dental pulp stem cells (DPSCs) from different age groups before and after induction of osteogenic differentiation. In addition, we also wanted to check their correlation with the degree of osteogenic differentiation. Methods: Human primary DPSCs from three age groups (milk tooth (SHEDs), 7–12 years old; young DPSCs (yDPSCs), 20–40 years old; old DPSCs (oDPSCs), 60+ years old) were characterized for mesenchymal stem cell (MSC) markers. DPSCs were subjected to osteogenic differentiation and functional staining. Gene expression levels were analyzed by qRT-PCR. Surface receptor analysis was done by flow cytometry. Comparative protein levels were evaluated by ELISA. Results: All SHEDs, yDPSCs, and oDPSCs were found to be expressing mesenchymal stem cell markers. SHEDs showed more mineralization than yDPSCs and oDPSCs after osteogenic induction. SHEDs exhibited higher expression of PTH and PTH1R before and after osteogenic induction, and after osteogenic induction, SHEDs showed more expression for RUNX2, ALPL, and OCN. Higher levels of PTH were observed in SHEDs and yDPSCs, and the number of PTH1R positive cells was relatively lower in yDPSCs and oDPSCs than in SHEDs. After osteogenic induction, SHEDs were superior in the secretion of OPG, and the secretions of ALPL and PTH and the number of PTH1R positive cells were relatively low in the oDPSCs. Conclusions: The therapeutic quality of dental pulp stem cells is largely based on their ability to retain their stemness characteristics. This study emphasizes the criterion of aging, which affects the secretion of PTH by these cells, which in turn attenuates their osteogenic potential. MDPI 2021-04-27 /pmc/articles/PMC8144966/ /pubmed/33925324 http://dx.doi.org/10.3390/jpm11050349 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bhandi, Shilpa
Alkahtani, Ahmed
Reda, Rodolfo
Mashyakhy, Mohammed
Boreak, Nezar
Maganur, Prabhadevi C.
Vishwanathaiah, Satish
Mehta, Deepak
Vyas, Nishant
Patil, Vikrant
Raj, A. Thirumal
Testarelli, Luca
Patil, Shankargouda
Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
title Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
title_full Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
title_fullStr Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
title_full_unstemmed Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
title_short Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
title_sort parathyroid hormone secretion and receptor expression determine the age-related degree of osteogenic differentiation in dental pulp stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144966/
https://www.ncbi.nlm.nih.gov/pubmed/33925324
http://dx.doi.org/10.3390/jpm11050349
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