Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar

The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronega...

Descripción completa

Detalles Bibliográficos
Autores principales: Touloudi, Antonia, Valiakos, George, Cawthraw, Shaun, Kostoulas, Polychronis, Gortázar, Christian, Boadella, Mariana, Giannakopoulos, Alexios, Birtsas, Periklis, Sofia, Marina, Athanasiou, Labrini V., Satra, Maria, Athanasakopoulou, Zoi, Kantere, Maria, Spyrou, Vassiliki, Petrovska, Liljana, Billinis, Charalambos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144985/
https://www.ncbi.nlm.nih.gov/pubmed/33922494
http://dx.doi.org/10.3390/microorganisms9050904
_version_ 1783697074594775040
author Touloudi, Antonia
Valiakos, George
Cawthraw, Shaun
Kostoulas, Polychronis
Gortázar, Christian
Boadella, Mariana
Giannakopoulos, Alexios
Birtsas, Periklis
Sofia, Marina
Athanasiou, Labrini V.
Satra, Maria
Athanasakopoulou, Zoi
Kantere, Maria
Spyrou, Vassiliki
Petrovska, Liljana
Billinis, Charalambos
author_facet Touloudi, Antonia
Valiakos, George
Cawthraw, Shaun
Kostoulas, Polychronis
Gortázar, Christian
Boadella, Mariana
Giannakopoulos, Alexios
Birtsas, Periklis
Sofia, Marina
Athanasiou, Labrini V.
Satra, Maria
Athanasakopoulou, Zoi
Kantere, Maria
Spyrou, Vassiliki
Petrovska, Liljana
Billinis, Charalambos
author_sort Touloudi, Antonia
collection PubMed
description The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar.
format Online
Article
Text
id pubmed-8144985
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81449852021-05-26 Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar Touloudi, Antonia Valiakos, George Cawthraw, Shaun Kostoulas, Polychronis Gortázar, Christian Boadella, Mariana Giannakopoulos, Alexios Birtsas, Periklis Sofia, Marina Athanasiou, Labrini V. Satra, Maria Athanasakopoulou, Zoi Kantere, Maria Spyrou, Vassiliki Petrovska, Liljana Billinis, Charalambos Microorganisms Article The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar. MDPI 2021-04-23 /pmc/articles/PMC8144985/ /pubmed/33922494 http://dx.doi.org/10.3390/microorganisms9050904 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Touloudi, Antonia
Valiakos, George
Cawthraw, Shaun
Kostoulas, Polychronis
Gortázar, Christian
Boadella, Mariana
Giannakopoulos, Alexios
Birtsas, Periklis
Sofia, Marina
Athanasiou, Labrini V.
Satra, Maria
Athanasakopoulou, Zoi
Kantere, Maria
Spyrou, Vassiliki
Petrovska, Liljana
Billinis, Charalambos
Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar
title Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar
title_full Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar
title_fullStr Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar
title_full_unstemmed Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar
title_short Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis in Wild Boar
title_sort development of a multiplex bead assay for simultaneous serodiagnosis of antibodies against mycobacterium bovis, brucella suis, and trichinella spiralis in wild boar
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144985/
https://www.ncbi.nlm.nih.gov/pubmed/33922494
http://dx.doi.org/10.3390/microorganisms9050904
work_keys_str_mv AT touloudiantonia developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT valiakosgeorge developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT cawthrawshaun developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT kostoulaspolychronis developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT gortazarchristian developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT boadellamariana developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT giannakopoulosalexios developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT birtsasperiklis developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT sofiamarina developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT athanasioulabriniv developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT satramaria developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT athanasakopoulouzoi developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT kanteremaria developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT spyrouvassiliki developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT petrovskaliljana developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar
AT billinischaralambos developmentofamultiplexbeadassayforsimultaneousserodiagnosisofantibodiesagainstmycobacteriumbovisbrucellasuisandtrichinellaspiralisinwildboar

Ejemplares similares