Cargando…

Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System

Regenerative medicine and stem cells could prove to be an effective solution to the problem of treating heart failure caused by ischemic heart disease. However, further studies on the understanding of the processes which occur during the regeneration of damaged tissue are needed. Microfluidic system...

Descripción completa

Detalles Bibliográficos
Autores principales: Kobuszewska, Anna, Kolodziejek, Dominik, Wojasinski, Michal, Ciach, Tomasz, Brzozka, Zbigniew, Jastrzebska, Elzbieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145317/
https://www.ncbi.nlm.nih.gov/pubmed/33922423
http://dx.doi.org/10.3390/bios11050131
_version_ 1783697145732268032
author Kobuszewska, Anna
Kolodziejek, Dominik
Wojasinski, Michal
Ciach, Tomasz
Brzozka, Zbigniew
Jastrzebska, Elzbieta
author_facet Kobuszewska, Anna
Kolodziejek, Dominik
Wojasinski, Michal
Ciach, Tomasz
Brzozka, Zbigniew
Jastrzebska, Elzbieta
author_sort Kobuszewska, Anna
collection PubMed
description Regenerative medicine and stem cells could prove to be an effective solution to the problem of treating heart failure caused by ischemic heart disease. However, further studies on the understanding of the processes which occur during the regeneration of damaged tissue are needed. Microfluidic systems, which provide conditions similar to in vivo, could be useful tools for the development of new therapies using stem cells. We investigated how mesenchymal stem cells (MSCs) affect the metabolic activity of cardiac cells (rat cardiomyoblasts and human cardiomyocytes) incubated with a potent uncoupler of mitochondrial oxidative phosphorylation under microfluidic conditions. A cyanide p-trifluoromethoxyphenylhydrazone (FCCP) was used to mimic disfunctions of mitochondria of cardiac cells. The study was performed in a microfluidic system integrated with nanofiber mats made of poly-l-lactid acid (PLLA) or polyurethane (PU). The microsystem geometry allows four different cell cultures to be conducted under different conditions (which we called: normal, abnormal—as both a mono- and co-culture). Metabolic activity of the cells, based on the bioluminescence assay, was assessed in the culture’s performed in the microsystem. It was proved that stem cells increased metabolic activity of cardiac cells maintained with FCCP.
format Online
Article
Text
id pubmed-8145317
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81453172021-05-26 Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System Kobuszewska, Anna Kolodziejek, Dominik Wojasinski, Michal Ciach, Tomasz Brzozka, Zbigniew Jastrzebska, Elzbieta Biosensors (Basel) Article Regenerative medicine and stem cells could prove to be an effective solution to the problem of treating heart failure caused by ischemic heart disease. However, further studies on the understanding of the processes which occur during the regeneration of damaged tissue are needed. Microfluidic systems, which provide conditions similar to in vivo, could be useful tools for the development of new therapies using stem cells. We investigated how mesenchymal stem cells (MSCs) affect the metabolic activity of cardiac cells (rat cardiomyoblasts and human cardiomyocytes) incubated with a potent uncoupler of mitochondrial oxidative phosphorylation under microfluidic conditions. A cyanide p-trifluoromethoxyphenylhydrazone (FCCP) was used to mimic disfunctions of mitochondria of cardiac cells. The study was performed in a microfluidic system integrated with nanofiber mats made of poly-l-lactid acid (PLLA) or polyurethane (PU). The microsystem geometry allows four different cell cultures to be conducted under different conditions (which we called: normal, abnormal—as both a mono- and co-culture). Metabolic activity of the cells, based on the bioluminescence assay, was assessed in the culture’s performed in the microsystem. It was proved that stem cells increased metabolic activity of cardiac cells maintained with FCCP. MDPI 2021-04-23 /pmc/articles/PMC8145317/ /pubmed/33922423 http://dx.doi.org/10.3390/bios11050131 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kobuszewska, Anna
Kolodziejek, Dominik
Wojasinski, Michal
Ciach, Tomasz
Brzozka, Zbigniew
Jastrzebska, Elzbieta
Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System
title Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System
title_full Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System
title_fullStr Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System
title_full_unstemmed Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System
title_short Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System
title_sort study of stem cells influence on cardiac cells cultured with a cyanide-p-trifluoromethoxyphenylhydrazone in organ-on-a-chip system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145317/
https://www.ncbi.nlm.nih.gov/pubmed/33922423
http://dx.doi.org/10.3390/bios11050131
work_keys_str_mv AT kobuszewskaanna studyofstemcellsinfluenceoncardiaccellsculturedwithacyanideptrifluoromethoxyphenylhydrazoneinorganonachipsystem
AT kolodziejekdominik studyofstemcellsinfluenceoncardiaccellsculturedwithacyanideptrifluoromethoxyphenylhydrazoneinorganonachipsystem
AT wojasinskimichal studyofstemcellsinfluenceoncardiaccellsculturedwithacyanideptrifluoromethoxyphenylhydrazoneinorganonachipsystem
AT ciachtomasz studyofstemcellsinfluenceoncardiaccellsculturedwithacyanideptrifluoromethoxyphenylhydrazoneinorganonachipsystem
AT brzozkazbigniew studyofstemcellsinfluenceoncardiaccellsculturedwithacyanideptrifluoromethoxyphenylhydrazoneinorganonachipsystem
AT jastrzebskaelzbieta studyofstemcellsinfluenceoncardiaccellsculturedwithacyanideptrifluoromethoxyphenylhydrazoneinorganonachipsystem