Improving Contagion and Horizontal Transmission of Entomopathogenic Fungi by the White-Spotted Longicorn Beetle, Anoplophora malasiaca, with Help of Contact Sex Pheromone

SIMPLE SUMMARY: The white-spotted longicorn beetle, Anoplophora malasiaca, is one of the most destructive pests of many fruits and street trees. Effective controls are needed because the effect of marketed insecticides is limited. Entomopathogenic fungi offer a solution, combination with the beetles’ contact sex pheromone. The surface of the female body is covered with contact sex pheromone, which we extracted. Males held onto a glass model coated with female extract for 5 h, but males held onto one without extract for <0.3 h. Males that held onto coated model, attached to fabric impregnated with an entomopathogenic fungus, Beauveria brongniartii, picked up significant fungi. The fungi were then transferred to females during mating. Our results indicate that a combination of contact pheromone with a pathogen could improve entomopathogenic infection of both male and female beetles. ABSTRACT: The white-spotted longicorn beetle, Anoplophora malasiaca, is one of the most destructive pests of horticultural crops and street trees. Effective controls are needed because the effect of marketed insecticides is limited. Entomopathogenic fungi offer a solution, and improving the rate of infection would be a breakthrough in this beetle’s control. The combination of pathogenic fungi and the beetle’s contact sex pheromone was suggested. The surface of the female body is covered with contact sex pheromone, which elicit male mating behavior. To develop a method for the practical control of this beetle, we evaluated the arrestant activity of female extract containing contact pheromone coated on a black glass model. Males presented with a coated model held on for 5 h (mean) during an 8-h experiment. In contrast, males presented with a control model held on for <0.3 h. Males that held onto coated models attached to fabric impregnated with conidia of the fungus Beauveria brongniartii picked up much conidia, which they then passed on to females during mating.