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Microfluidics for Peptidomics, Proteomics, and Cell Analysis

Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1–100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant...

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Autores principales: Vitorino, Rui, Guedes, Sofia, da Costa, João Pinto, Kašička, Václav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145566/
https://www.ncbi.nlm.nih.gov/pubmed/33925983
http://dx.doi.org/10.3390/nano11051118
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author Vitorino, Rui
Guedes, Sofia
da Costa, João Pinto
Kašička, Václav
author_facet Vitorino, Rui
Guedes, Sofia
da Costa, João Pinto
Kašička, Václav
author_sort Vitorino, Rui
collection PubMed
description Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1–100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant to separation, analysis, and detection. Currently, microfluidic devices play an important role in many biological, chemical, physical, biotechnological and engineering applications. There are numerous ways to fabricate the necessary microchannels and integrate them into microfluidic platforms. In peptidomics and proteomics, microfluidics is often used in combination with mass spectrometric (MS) analysis. This review provides an overview of using microfluidic systems for peptidomics, proteomics and cell analysis. The application of microfluidics in combination with MS detection and other novel techniques to answer clinical questions is also discussed in the context of disease diagnosis and therapy. Recent developments and applications of capillary and microchip (electro)separation methods in proteomic and peptidomic analysis are summarized. The state of the art of microchip platforms for cell sorting and single-cell analysis is also discussed. Advances in detection methods are reported, and new applications in proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices and determination of their physicochemical parameters are highlighted.
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spelling pubmed-81455662021-05-26 Microfluidics for Peptidomics, Proteomics, and Cell Analysis Vitorino, Rui Guedes, Sofia da Costa, João Pinto Kašička, Václav Nanomaterials (Basel) Review Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1–100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant to separation, analysis, and detection. Currently, microfluidic devices play an important role in many biological, chemical, physical, biotechnological and engineering applications. There are numerous ways to fabricate the necessary microchannels and integrate them into microfluidic platforms. In peptidomics and proteomics, microfluidics is often used in combination with mass spectrometric (MS) analysis. This review provides an overview of using microfluidic systems for peptidomics, proteomics and cell analysis. The application of microfluidics in combination with MS detection and other novel techniques to answer clinical questions is also discussed in the context of disease diagnosis and therapy. Recent developments and applications of capillary and microchip (electro)separation methods in proteomic and peptidomic analysis are summarized. The state of the art of microchip platforms for cell sorting and single-cell analysis is also discussed. Advances in detection methods are reported, and new applications in proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices and determination of their physicochemical parameters are highlighted. MDPI 2021-04-26 /pmc/articles/PMC8145566/ /pubmed/33925983 http://dx.doi.org/10.3390/nano11051118 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Vitorino, Rui
Guedes, Sofia
da Costa, João Pinto
Kašička, Václav
Microfluidics for Peptidomics, Proteomics, and Cell Analysis
title Microfluidics for Peptidomics, Proteomics, and Cell Analysis
title_full Microfluidics for Peptidomics, Proteomics, and Cell Analysis
title_fullStr Microfluidics for Peptidomics, Proteomics, and Cell Analysis
title_full_unstemmed Microfluidics for Peptidomics, Proteomics, and Cell Analysis
title_short Microfluidics for Peptidomics, Proteomics, and Cell Analysis
title_sort microfluidics for peptidomics, proteomics, and cell analysis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145566/
https://www.ncbi.nlm.nih.gov/pubmed/33925983
http://dx.doi.org/10.3390/nano11051118
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