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Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva

A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary id...

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Autores principales: Taghipour, Simin, Abastabar, Mahdi, Piri, Fahimeh, Aboualigalehdari, Elham, Jabbari, Mohammad Reza, Zarrinfar, Hossein, Nouripour-Sisakht, Sadegh, Mohammadi, Rasoul, Ahmadi, Bahram, Ansari, Saham, Katiraee, Farzad, Niknejad, Farhad, Didehdar, Mojtaba, Nazeri, Mehdi, Makimura, Koichi, Rezaei-Matehkolaei, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145706/
https://www.ncbi.nlm.nih.gov/pubmed/33925095
http://dx.doi.org/10.3390/jof7050345
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author Taghipour, Simin
Abastabar, Mahdi
Piri, Fahimeh
Aboualigalehdari, Elham
Jabbari, Mohammad Reza
Zarrinfar, Hossein
Nouripour-Sisakht, Sadegh
Mohammadi, Rasoul
Ahmadi, Bahram
Ansari, Saham
Katiraee, Farzad
Niknejad, Farhad
Didehdar, Mojtaba
Nazeri, Mehdi
Makimura, Koichi
Rezaei-Matehkolaei, Ali
author_facet Taghipour, Simin
Abastabar, Mahdi
Piri, Fahimeh
Aboualigalehdari, Elham
Jabbari, Mohammad Reza
Zarrinfar, Hossein
Nouripour-Sisakht, Sadegh
Mohammadi, Rasoul
Ahmadi, Bahram
Ansari, Saham
Katiraee, Farzad
Niknejad, Farhad
Didehdar, Mojtaba
Nazeri, Mehdi
Makimura, Koichi
Rezaei-Matehkolaei, Ali
author_sort Taghipour, Simin
collection PubMed
description A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary identification of isolates was carried out by amplification and MvaI restriction fragment length polymorphism (RFLP) of the internal transcribed spacers regions of ribosomal DNA (ITS-rDNA). The identifications, especially in the cases of isolates with unknown RFLP patterns, were confirmed by sequencing of the ITS-rDNA region. As a result, 256 isolates were recovered. The isolation rate was higher in soils with pH range 7.1–8.0, collected from animal habitats (n = 78; 34%) and parks and gardens (n = 75; 32%), geographically from Mazandaran Province (n = 115; 49.5%) and seasonally in the spring (n = 129; 50.4%), all of which were statistically significant (p < 0.05). The dermatophytes comprising five species of the two genera, viz., Nannizzia fulva (n = 214), N. gypsea (n = 34), Arthroderma quadrifidum (n = 5), A. gertleri (n = 2) and A. tuberculatum (n = 1), were isolated. The geophilic dermatophytes occurred in various soils from different parts of Iran; however, surprisingly, N. fulva emerged as the dominant species, outnumbering the common geophilic species of N. gypsea. For the definitive identification of soil inhabitant dermatophytes, DNA-based identification is strongly recommended.
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spelling pubmed-81457062021-05-26 Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva Taghipour, Simin Abastabar, Mahdi Piri, Fahimeh Aboualigalehdari, Elham Jabbari, Mohammad Reza Zarrinfar, Hossein Nouripour-Sisakht, Sadegh Mohammadi, Rasoul Ahmadi, Bahram Ansari, Saham Katiraee, Farzad Niknejad, Farhad Didehdar, Mojtaba Nazeri, Mehdi Makimura, Koichi Rezaei-Matehkolaei, Ali J Fungi (Basel) Article A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary identification of isolates was carried out by amplification and MvaI restriction fragment length polymorphism (RFLP) of the internal transcribed spacers regions of ribosomal DNA (ITS-rDNA). The identifications, especially in the cases of isolates with unknown RFLP patterns, were confirmed by sequencing of the ITS-rDNA region. As a result, 256 isolates were recovered. The isolation rate was higher in soils with pH range 7.1–8.0, collected from animal habitats (n = 78; 34%) and parks and gardens (n = 75; 32%), geographically from Mazandaran Province (n = 115; 49.5%) and seasonally in the spring (n = 129; 50.4%), all of which were statistically significant (p < 0.05). The dermatophytes comprising five species of the two genera, viz., Nannizzia fulva (n = 214), N. gypsea (n = 34), Arthroderma quadrifidum (n = 5), A. gertleri (n = 2) and A. tuberculatum (n = 1), were isolated. The geophilic dermatophytes occurred in various soils from different parts of Iran; however, surprisingly, N. fulva emerged as the dominant species, outnumbering the common geophilic species of N. gypsea. For the definitive identification of soil inhabitant dermatophytes, DNA-based identification is strongly recommended. MDPI 2021-04-28 /pmc/articles/PMC8145706/ /pubmed/33925095 http://dx.doi.org/10.3390/jof7050345 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Taghipour, Simin
Abastabar, Mahdi
Piri, Fahimeh
Aboualigalehdari, Elham
Jabbari, Mohammad Reza
Zarrinfar, Hossein
Nouripour-Sisakht, Sadegh
Mohammadi, Rasoul
Ahmadi, Bahram
Ansari, Saham
Katiraee, Farzad
Niknejad, Farhad
Didehdar, Mojtaba
Nazeri, Mehdi
Makimura, Koichi
Rezaei-Matehkolaei, Ali
Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
title Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
title_full Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
title_fullStr Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
title_full_unstemmed Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
title_short Diversity of Geophilic Dermatophytes Species in the Soils of Iran; The Significant Preponderance of Nannizzia fulva
title_sort diversity of geophilic dermatophytes species in the soils of iran; the significant preponderance of nannizzia fulva
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145706/
https://www.ncbi.nlm.nih.gov/pubmed/33925095
http://dx.doi.org/10.3390/jof7050345
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