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Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology

The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanopartic...

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Detalles Bibliográficos
Autores principales: El-Beyrouthy, Joyce, Freeman, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145864/
https://www.ncbi.nlm.nih.gov/pubmed/33925756
http://dx.doi.org/10.3390/membranes11050319
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author El-Beyrouthy, Joyce
Freeman, Eric
author_facet El-Beyrouthy, Joyce
Freeman, Eric
author_sort El-Beyrouthy, Joyce
collection PubMed
description The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanoparticle cytotoxicity. Characterization is often accomplished by recreating model membranes that approximate the structure of cellular membranes in a controlled environment, formed using self-assembly principles. The selected method for membrane creation influences the properties of the membrane assembly, including their response to electric fields used for characterizing transmembrane exchanges. When these self-assembled model membranes are combined with electrophysiology, it is possible to exploit their non-physiological mechanics to enable additional measurements of membrane interactions and phenomena. This review describes several common model membranes including liposomes, pore-spanning membranes, solid supported membranes, and emulsion-based membranes, emphasizing their varying structure due to the selected mode of production. Next, electrophysiology techniques that exploit these structures are discussed, including conductance measurements, electrowetting and electrocompression analysis, and electroimpedance spectroscopy. The focus of this review is linking each membrane assembly technique to the properties of the resulting membrane, discussing how these properties enable alternative electrophysiological approaches to measuring membrane characteristics and interactions.
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spelling pubmed-81458642021-05-26 Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology El-Beyrouthy, Joyce Freeman, Eric Membranes (Basel) Review The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanoparticle cytotoxicity. Characterization is often accomplished by recreating model membranes that approximate the structure of cellular membranes in a controlled environment, formed using self-assembly principles. The selected method for membrane creation influences the properties of the membrane assembly, including their response to electric fields used for characterizing transmembrane exchanges. When these self-assembled model membranes are combined with electrophysiology, it is possible to exploit their non-physiological mechanics to enable additional measurements of membrane interactions and phenomena. This review describes several common model membranes including liposomes, pore-spanning membranes, solid supported membranes, and emulsion-based membranes, emphasizing their varying structure due to the selected mode of production. Next, electrophysiology techniques that exploit these structures are discussed, including conductance measurements, electrowetting and electrocompression analysis, and electroimpedance spectroscopy. The focus of this review is linking each membrane assembly technique to the properties of the resulting membrane, discussing how these properties enable alternative electrophysiological approaches to measuring membrane characteristics and interactions. MDPI 2021-04-27 /pmc/articles/PMC8145864/ /pubmed/33925756 http://dx.doi.org/10.3390/membranes11050319 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
El-Beyrouthy, Joyce
Freeman, Eric
Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
title Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
title_full Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
title_fullStr Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
title_full_unstemmed Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
title_short Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
title_sort characterizing the structure and interactions of model lipid membranes using electrophysiology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145864/
https://www.ncbi.nlm.nih.gov/pubmed/33925756
http://dx.doi.org/10.3390/membranes11050319
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