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In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)

Three pathogenic fungi of blueberry (Vaccinium spp.) responsible for dieback disease, identified as Pestalotiopsis clavispora, Colletotrichum gloeosporioides and Lasiodiplodia pseudotheobromae, were isolated in the northwestern region of the state of Michoacán, Mexico. The mycelial growth in vitro o...

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Autores principales: Hernández-Ceja, Abraham, Loeza-Lara, Pedro Damián, Espinosa-García, Francisco Javier, García-Rodríguez, Yolanda M., Medina-Medrano, José Roberto, Gutiérrez-Hernández, Germán Fernando, Ceja-Torres, Luis Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145869/
https://www.ncbi.nlm.nih.gov/pubmed/33922510
http://dx.doi.org/10.3390/plants10050852
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author Hernández-Ceja, Abraham
Loeza-Lara, Pedro Damián
Espinosa-García, Francisco Javier
García-Rodríguez, Yolanda M.
Medina-Medrano, José Roberto
Gutiérrez-Hernández, Germán Fernando
Ceja-Torres, Luis Fernando
author_facet Hernández-Ceja, Abraham
Loeza-Lara, Pedro Damián
Espinosa-García, Francisco Javier
García-Rodríguez, Yolanda M.
Medina-Medrano, José Roberto
Gutiérrez-Hernández, Germán Fernando
Ceja-Torres, Luis Fernando
author_sort Hernández-Ceja, Abraham
collection PubMed
description Three pathogenic fungi of blueberry (Vaccinium spp.) responsible for dieback disease, identified as Pestalotiopsis clavispora, Colletotrichum gloeosporioides and Lasiodiplodia pseudotheobromae, were isolated in the northwestern region of the state of Michoacán, Mexico. The mycelial growth in vitro of these fungi was inhibited by extracts from Lantana hirta, Argemone ochroleuca and Adenophyllum porophyllum, medicinal plants collected in Sahuayo, Michoacán, Mexico. The extracts showed different degrees of inhibition; the most effective were: M5L extract from L. hirta and M6LFr extract from A. ochroleuca, both of which inhibited 100% of the mycelial growth of P. clavispora and C. gloeosporioides; and M4LS extract from A. porophyllum, which inhibited 100% of the mycelial growth of the three pathogens. The extracts were fractionated by thin layer and column chromatography, and the most active fractions were analyzed by gas chromatography-mass spectrometry. The major compounds identified in L. hirta extract were Phytol and α-Sitosterol. The compounds identified in A. ochroleuca were Toluene and Benzene, 1,3-bis(3-phenoxyphenoxy)-. In A. porophyllum, the compound identified was Hexanedioic acid, bis(2-ethylhexyl) ester. These results show the potential of L. hirta, A. ochroleuca and A. porophyllum as a source of antifungal compounds.
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spelling pubmed-81458692021-05-26 In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.) Hernández-Ceja, Abraham Loeza-Lara, Pedro Damián Espinosa-García, Francisco Javier García-Rodríguez, Yolanda M. Medina-Medrano, José Roberto Gutiérrez-Hernández, Germán Fernando Ceja-Torres, Luis Fernando Plants (Basel) Article Three pathogenic fungi of blueberry (Vaccinium spp.) responsible for dieback disease, identified as Pestalotiopsis clavispora, Colletotrichum gloeosporioides and Lasiodiplodia pseudotheobromae, were isolated in the northwestern region of the state of Michoacán, Mexico. The mycelial growth in vitro of these fungi was inhibited by extracts from Lantana hirta, Argemone ochroleuca and Adenophyllum porophyllum, medicinal plants collected in Sahuayo, Michoacán, Mexico. The extracts showed different degrees of inhibition; the most effective were: M5L extract from L. hirta and M6LFr extract from A. ochroleuca, both of which inhibited 100% of the mycelial growth of P. clavispora and C. gloeosporioides; and M4LS extract from A. porophyllum, which inhibited 100% of the mycelial growth of the three pathogens. The extracts were fractionated by thin layer and column chromatography, and the most active fractions were analyzed by gas chromatography-mass spectrometry. The major compounds identified in L. hirta extract were Phytol and α-Sitosterol. The compounds identified in A. ochroleuca were Toluene and Benzene, 1,3-bis(3-phenoxyphenoxy)-. In A. porophyllum, the compound identified was Hexanedioic acid, bis(2-ethylhexyl) ester. These results show the potential of L. hirta, A. ochroleuca and A. porophyllum as a source of antifungal compounds. MDPI 2021-04-23 /pmc/articles/PMC8145869/ /pubmed/33922510 http://dx.doi.org/10.3390/plants10050852 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Ceja, Abraham
Loeza-Lara, Pedro Damián
Espinosa-García, Francisco Javier
García-Rodríguez, Yolanda M.
Medina-Medrano, José Roberto
Gutiérrez-Hernández, Germán Fernando
Ceja-Torres, Luis Fernando
In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)
title In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)
title_full In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)
title_fullStr In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)
title_full_unstemmed In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)
title_short In Vitro Antifungal Activity of Plant Extracts on Pathogenic Fungi of Blueberry (Vaccinium sp.)
title_sort in vitro antifungal activity of plant extracts on pathogenic fungi of blueberry (vaccinium sp.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145869/
https://www.ncbi.nlm.nih.gov/pubmed/33922510
http://dx.doi.org/10.3390/plants10050852
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