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Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics

We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process and integrating all relevant software...

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Detalles Bibliográficos
Autores principales: Bergès, Cécilia, Cahoreau, Edern, Millard, Pierre, Enjalbert, Brice, Dinclaux, Mickael, Heuillet, Maud, Kulyk, Hanna, Gales, Lara, Butin, Noémie, Chazalviel, Maxime, Palama, Tony, Guionnet, Matthieu, Sokol, Sergueï, Peyriga, Lindsay, Bellvert, Floriant, Heux, Stéphanie, Portais, Jean-Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145925/
https://www.ncbi.nlm.nih.gov/pubmed/33926117
http://dx.doi.org/10.3390/metabo11050271
Descripción
Sumario:We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process and integrating all relevant software tools. This workflow allowed us to obtain highly detailed maps of carbon fluxes in the central carbon metabolism in a fully automated manner. It was applied to investigate the glucose fluxotypes of 180 Escherichia coli strains deleted for y-genes. Since the products of these y-genes potentially play a role in a variety of metabolic processes, the experiments were designed to be agnostic as to their potential metabolic impact. The obtained data highlight the robustness of E. coli’s central metabolism to y-gene deletion. For two y-genes, deletion resulted in significant changes in carbon and energy fluxes, demonstrating the involvement of the corresponding y-gene products in metabolic function or regulation. This work also introduces novel metrics to measure the actual scope and quality of high-throughput fluxomics investigations.