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Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies

The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences betwe...

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Autores principales: Mazur, Monika, Wojciechowska, Daria, Sitkiewicz, Ewa, Malinowska, Agata, Świderska, Bianka, Kmita, Hanna, Wojtkowska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145953/
https://www.ncbi.nlm.nih.gov/pubmed/33923051
http://dx.doi.org/10.3390/genes12050638
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author Mazur, Monika
Wojciechowska, Daria
Sitkiewicz, Ewa
Malinowska, Agata
Świderska, Bianka
Kmita, Hanna
Wojtkowska, Małgorzata
author_facet Mazur, Monika
Wojciechowska, Daria
Sitkiewicz, Ewa
Malinowska, Agata
Świderska, Bianka
Kmita, Hanna
Wojtkowska, Małgorzata
author_sort Mazur, Monika
collection PubMed
description The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences between the mitochondria isolated from the slime mold regarding its early developmental stages induced by starvation, namely the unicellular (U), aggregation (A) and streams (S) stages, at the bioenergetic and proteome levels. We measured the oxygen consumption of intact cells using the Clarke electrode and observed a distinct decrease in mitochondrial coupling capacity for stage S cells and a decrease in mitochondrial coupling efficiency for stage A and S cells. We also found changes in spare respiratory capacity. We performed a wide comparative proteomic study. During the transition from the unicellular stage to the multicellular stage, important proteomic differences occurred in stages A and S relating to the proteins of the main mitochondrial functional groups, showing characteristic tendencies that could be associated with their ongoing adaptation to starvation following cell reprogramming during the switch to gluconeogenesis. We suggest that the main mitochondrial processes are downregulated during the early developmental stages, although this needs to be verified by extending analogous studies to the next slime mold life cycle stages.
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spelling pubmed-81459532021-05-26 Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies Mazur, Monika Wojciechowska, Daria Sitkiewicz, Ewa Malinowska, Agata Świderska, Bianka Kmita, Hanna Wojtkowska, Małgorzata Genes (Basel) Article The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences between the mitochondria isolated from the slime mold regarding its early developmental stages induced by starvation, namely the unicellular (U), aggregation (A) and streams (S) stages, at the bioenergetic and proteome levels. We measured the oxygen consumption of intact cells using the Clarke electrode and observed a distinct decrease in mitochondrial coupling capacity for stage S cells and a decrease in mitochondrial coupling efficiency for stage A and S cells. We also found changes in spare respiratory capacity. We performed a wide comparative proteomic study. During the transition from the unicellular stage to the multicellular stage, important proteomic differences occurred in stages A and S relating to the proteins of the main mitochondrial functional groups, showing characteristic tendencies that could be associated with their ongoing adaptation to starvation following cell reprogramming during the switch to gluconeogenesis. We suggest that the main mitochondrial processes are downregulated during the early developmental stages, although this needs to be verified by extending analogous studies to the next slime mold life cycle stages. MDPI 2021-04-25 /pmc/articles/PMC8145953/ /pubmed/33923051 http://dx.doi.org/10.3390/genes12050638 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mazur, Monika
Wojciechowska, Daria
Sitkiewicz, Ewa
Malinowska, Agata
Świderska, Bianka
Kmita, Hanna
Wojtkowska, Małgorzata
Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies
title Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies
title_full Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies
title_fullStr Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies
title_full_unstemmed Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies
title_short Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies
title_sort mitochondrial processes during early development of dictyostelium discoideum: from bioenergetic to proteomic studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145953/
https://www.ncbi.nlm.nih.gov/pubmed/33923051
http://dx.doi.org/10.3390/genes12050638
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