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Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity

The histopathologic diagnosis of prostate cancer (PCa) from biopsies is a current challenge if double or triple staining is needed. Therefore, there is an urgent need for development of a new reliable biomarker to diagnose PCa patients. We aimed to explore and compare the expression of TMTC4 in PCa...

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Autores principales: Makboul, Rania, Abdelkawi, Islam F., Badary, Dalia M., Hussein, Mahmoud R. A., Rhim, Johng S., Toraih, Eman A., Zerfaoui, Mourad, Abd Elmageed, Zakaria Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146280/
https://www.ncbi.nlm.nih.gov/pubmed/33925440
http://dx.doi.org/10.3390/cells10051029
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author Makboul, Rania
Abdelkawi, Islam F.
Badary, Dalia M.
Hussein, Mahmoud R. A.
Rhim, Johng S.
Toraih, Eman A.
Zerfaoui, Mourad
Abd Elmageed, Zakaria Y.
author_facet Makboul, Rania
Abdelkawi, Islam F.
Badary, Dalia M.
Hussein, Mahmoud R. A.
Rhim, Johng S.
Toraih, Eman A.
Zerfaoui, Mourad
Abd Elmageed, Zakaria Y.
author_sort Makboul, Rania
collection PubMed
description The histopathologic diagnosis of prostate cancer (PCa) from biopsies is a current challenge if double or triple staining is needed. Therefore, there is an urgent need for development of a new reliable biomarker to diagnose PCa patients. We aimed to explore and compare the expression of TMTC4 in PCa cells and tissue specimens and evaluate its sensitivity and specificity. The expression of TMTC4 in PCa and normal prostate epithelial cells was determined by real-time PCR and Western blot analyses. Immunohistochemical (IHC) staining of TMTC4 was performed on tissues collected from PCa and benign prostatic hyperplasia (BPH). Our results show a high expression of TMTC4 on mRNA and protein levels in PCa versus BPH1 and normal cells (p < 0.05). IHC results show strong cytoplasmic expressions in PCa cases (p < 0.001) as compared to BPH cases. The overall accuracy as measured by the AUC was 1.0 (p < 0.001). The sensitivity and specificity of the protein were 100% and 96.6%, respectively. Taken together, we report a high TMTC4 expression in PCa cells and tissues and its ability to differentiate between PCa and BPH with high sensitivity and specificity. This finding can be carried over to clinical practice after its confirmation by further studies.
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spelling pubmed-81462802021-05-26 Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity Makboul, Rania Abdelkawi, Islam F. Badary, Dalia M. Hussein, Mahmoud R. A. Rhim, Johng S. Toraih, Eman A. Zerfaoui, Mourad Abd Elmageed, Zakaria Y. Cells Article The histopathologic diagnosis of prostate cancer (PCa) from biopsies is a current challenge if double or triple staining is needed. Therefore, there is an urgent need for development of a new reliable biomarker to diagnose PCa patients. We aimed to explore and compare the expression of TMTC4 in PCa cells and tissue specimens and evaluate its sensitivity and specificity. The expression of TMTC4 in PCa and normal prostate epithelial cells was determined by real-time PCR and Western blot analyses. Immunohistochemical (IHC) staining of TMTC4 was performed on tissues collected from PCa and benign prostatic hyperplasia (BPH). Our results show a high expression of TMTC4 on mRNA and protein levels in PCa versus BPH1 and normal cells (p < 0.05). IHC results show strong cytoplasmic expressions in PCa cases (p < 0.001) as compared to BPH cases. The overall accuracy as measured by the AUC was 1.0 (p < 0.001). The sensitivity and specificity of the protein were 100% and 96.6%, respectively. Taken together, we report a high TMTC4 expression in PCa cells and tissues and its ability to differentiate between PCa and BPH with high sensitivity and specificity. This finding can be carried over to clinical practice after its confirmation by further studies. MDPI 2021-04-27 /pmc/articles/PMC8146280/ /pubmed/33925440 http://dx.doi.org/10.3390/cells10051029 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Makboul, Rania
Abdelkawi, Islam F.
Badary, Dalia M.
Hussein, Mahmoud R. A.
Rhim, Johng S.
Toraih, Eman A.
Zerfaoui, Mourad
Abd Elmageed, Zakaria Y.
Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity
title Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity
title_full Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity
title_fullStr Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity
title_full_unstemmed Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity
title_short Transmembrane and Tetratricopeptide Repeat Containing 4 Is a Novel Diagnostic Marker for Prostate Cancer with High Specificity and Sensitivity
title_sort transmembrane and tetratricopeptide repeat containing 4 is a novel diagnostic marker for prostate cancer with high specificity and sensitivity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146280/
https://www.ncbi.nlm.nih.gov/pubmed/33925440
http://dx.doi.org/10.3390/cells10051029
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