Cargando…

MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’

The phenomenon of multi-carpel and multi-ovule exists in the grapevine cultivar ‘Xiangfei’, but the mechanism of ovule formation is seldom reported. In this study, we observed the ovule formation process by using ‘Xiangfei’ grapes. The role of the VvAG2 (VvAGAMOUS) gene in ovule formation was identi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yan, Liu, Zhenhua, Wu, Jiang, Hong, Liang, Liang, Jinjun, Ren, Yangmei, Guan, Pingyin, Hu, Jianfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146481/
https://www.ncbi.nlm.nih.gov/pubmed/33926100
http://dx.doi.org/10.3390/genes12050647
_version_ 1783697407307939840
author Wang, Yan
Liu, Zhenhua
Wu, Jiang
Hong, Liang
Liang, Jinjun
Ren, Yangmei
Guan, Pingyin
Hu, Jianfang
author_facet Wang, Yan
Liu, Zhenhua
Wu, Jiang
Hong, Liang
Liang, Jinjun
Ren, Yangmei
Guan, Pingyin
Hu, Jianfang
author_sort Wang, Yan
collection PubMed
description The phenomenon of multi-carpel and multi-ovule exists in the grapevine cultivar ‘Xiangfei’, but the mechanism of ovule formation is seldom reported. In this study, we observed the ovule formation process by using ‘Xiangfei’ grapes. The role of the VvAG2 (VvAGAMOUS) gene in ovule formation was identified, and we explored the relationship between VvAG2, VvSEP3(VvMADS4) and VvAGL11(VvMADS5) proteins. The results showed that the ovule primordium appeared when the inflorescence length of ‘Xiangfei’ grapes were 4–5 cm long; the relative expression levels of VvAG2, VvAGL11 and VvSEP3 genes were higher during ovule formation, and the expression levels of VvAG2 gene was the highest. Transgenic tomato (Solanum lycopersicum) plants expressing VvAG2 produced higher numbers of ovules and carpels than the wild type. Moreover, yeast two-hybrid and yeast three-hybrid experiments demonstrated that VvSEP3 acts as a bridge and interacts with VvAG2 and VvAGL11 proteins, respectively. Meanwhile, a homodimer can be formed between VvSEP3 and VvSEP3, but there was no interaction between VvAG2 and VvAGL11. These findings suggest that the VvAG2 gene is involved in the formation of ovules, and VvAG2/VvSEP3 together with VvAGL11/VvSEP3 can form a tetrameric complex. In summary, our data showed that VvAG2 along with VvSEP3 and VvAGL11 jointly regulate the ovule formation of ‘Xiangfei’ grapes.
format Online
Article
Text
id pubmed-8146481
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81464812021-05-26 MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’ Wang, Yan Liu, Zhenhua Wu, Jiang Hong, Liang Liang, Jinjun Ren, Yangmei Guan, Pingyin Hu, Jianfang Genes (Basel) Article The phenomenon of multi-carpel and multi-ovule exists in the grapevine cultivar ‘Xiangfei’, but the mechanism of ovule formation is seldom reported. In this study, we observed the ovule formation process by using ‘Xiangfei’ grapes. The role of the VvAG2 (VvAGAMOUS) gene in ovule formation was identified, and we explored the relationship between VvAG2, VvSEP3(VvMADS4) and VvAGL11(VvMADS5) proteins. The results showed that the ovule primordium appeared when the inflorescence length of ‘Xiangfei’ grapes were 4–5 cm long; the relative expression levels of VvAG2, VvAGL11 and VvSEP3 genes were higher during ovule formation, and the expression levels of VvAG2 gene was the highest. Transgenic tomato (Solanum lycopersicum) plants expressing VvAG2 produced higher numbers of ovules and carpels than the wild type. Moreover, yeast two-hybrid and yeast three-hybrid experiments demonstrated that VvSEP3 acts as a bridge and interacts with VvAG2 and VvAGL11 proteins, respectively. Meanwhile, a homodimer can be formed between VvSEP3 and VvSEP3, but there was no interaction between VvAG2 and VvAGL11. These findings suggest that the VvAG2 gene is involved in the formation of ovules, and VvAG2/VvSEP3 together with VvAGL11/VvSEP3 can form a tetrameric complex. In summary, our data showed that VvAG2 along with VvSEP3 and VvAGL11 jointly regulate the ovule formation of ‘Xiangfei’ grapes. MDPI 2021-04-26 /pmc/articles/PMC8146481/ /pubmed/33926100 http://dx.doi.org/10.3390/genes12050647 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yan
Liu, Zhenhua
Wu, Jiang
Hong, Liang
Liang, Jinjun
Ren, Yangmei
Guan, Pingyin
Hu, Jianfang
MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’
title MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’
title_full MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’
title_fullStr MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’
title_full_unstemmed MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’
title_short MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’
title_sort mads-box protein complex vvag2, vvsep3 and vvagl11 regulates the formation of ovules in vitis vinifera l. cv. ‘xiangfei’
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146481/
https://www.ncbi.nlm.nih.gov/pubmed/33926100
http://dx.doi.org/10.3390/genes12050647
work_keys_str_mv AT wangyan madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT liuzhenhua madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT wujiang madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT hongliang madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT liangjinjun madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT renyangmei madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT guanpingyin madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei
AT hujianfang madsboxproteincomplexvvag2vvsep3andvvagl11regulatestheformationofovulesinvitisviniferalcvxiangfei