Cargando…

An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples

Lysozymes are used in sterilisation, antisepsis, dairy additives, inflammation, and cancer. One transgenic goat line expressing high levels of human lysozyme (hLZ) in goat milk has been developed in China. Herein, we established an event-specific real-time polymerase chain reaction (real-time PCR) m...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xu, Wenting, Cui, Jinjie, Liu, Biao, Yang, Litao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146569/ https://www.ncbi.nlm.nih.gov/pubmed/33922422 http://dx.doi.org/10.3390/foods10050925

Ejemplares similares

Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM PRNP-Knockout Goat
por: Xu, Wenting, et al.
Publicado: (2022)

Lysozyme transgenic goats’ milk positively impacts intestinal cytokine expression and morphology
por: Cooper, Caitlin A., et al.
Publicado: (2011)

Evaluating the fitness of human lysozyme transgenic dairy goats: growth and reproductive traits
por: Jackson, Kathryn A., et al.
Publicado: (2010)

Consumption of pasteurized human lysozyme transgenic goats’ milk alters serum metabolite profile in young pigs
por: Brundige, Dottie R., et al.
Publicado: (2009)

Consuming Transgenic Goats' Milk Containing the Antimicrobial Protein Lysozyme Helps Resolve Diarrhea in Young Pigs
por: Cooper, Caitlin A., et al.
Publicado: (2013)

Effects of over-expression of TLR2 in transgenic goats on pathogen clearance and role of up-regulation of lysozyme secretion and infiltration of inflammatory cells
por: Deng, Shoulong, et al.
Publicado: (2012)

The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish
por: Hall, Chris, et al.
Publicado: (2007)

Novel Iodine-induced Cleavage Real-time PCR Assay for Accurate Quantification of Phosphorothioate Modified Sites in Bacterial DNA
por: Chen, Yi, et al.
Publicado: (2019)

Expression of Recombinant Human Lysozyme in Egg Whites of Transgenic Hens
por: Cao, Dainan, et al.
Publicado: (2015)

Event-specific detection of transgenic potato AV43-6-G7 using real-time and digital PCR methods
por: Gao, Hongwei, et al.
Publicado: (2016)

Microbiome Associated with Slovak Raw Goat Milk, Trace Minerals, and Vitamin E Content
por: Lauková, Andrea, et al.
Publicado: (2022)

Purification and Characterization of Recombinant Human Lysozyme from Eggs of Transgenic Chickens
por: Wu, Hanyu, et al.
Publicado: (2015)

Characterization of Bioactive Recombinant Human Lysozyme Expressed in Milk of Cloned Transgenic Cattle
por: Yang, Bin, et al.
Publicado: (2011)

In vitro PCR verification that lysozyme inhibits nucleic acid replication and transcription
por: Liu, Lu, et al.
Publicado: (2023)

Production of Transgenic-Cloned Pigs Expressing Large Quantities of Recombinant Human Lysozyme in Milk
por: Lu, Dan, et al.
Publicado: (2015)

Lysozyme Inhibitors as Tools for Lysozyme Profiling: Identification and Antibacterial Function of Lysozymes in the Hemolymph of the Blue Mussel
por: Vanderkelen, Lise, et al.
Publicado: (2023)

Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
por: Ahmed, Marwa A., et al.
Publicado: (2021)

Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR
por: Wareth, Gamal, et al.
Publicado: (2015)

Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat
por: Guo, Qinghong, et al.
Publicado: (2020)

Foll Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia
por: Tavassoli, Mousa, et al.
Publicado: (2013)

Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice
por: Huang, Yue-Jin, et al.
Publicado: (2005)

The goat β-casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection
por: Zhang, Ting, et al.
Publicado: (2019)

Large-scale production of functional human lysozyme from marker-free transgenic cloned cows
por: Lu, Dan, et al.
Publicado: (2016)

Statistical tools for transgene copy number estimation based on real-time PCR
por: Yuan, Joshua S, et al.
Publicado: (2007)

Real-time PCR for the detection of precise transgene copy number in durum wheat
por: Gadaleta, Agata, et al.
Publicado: (2011)

Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.)
por: Nageswara-Rao, Madhugiri, et al.
Publicado: (2013)

Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli
por: Carvalho, Eunice B, et al.
Publicado: (2012)

Transgenic goats producing an improved version of cetuximab in milk
por: Laible, Götz, et al.
Publicado: (2020)

A novel recombinant human plasminogen activator: Efficient expression and hereditary stability in transgenic goats and in vitro thrombolytic bioactivity in the milk of transgenic goats
por: He, Zhengyi, et al.
Publicado: (2018)

Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples
por: Masiero, Eva, et al.
Publicado: (2017)

Effect of Hen Genotype and Laying Time on Egg Quality and Albumen Lysozyme Content and Activity
por: Krawczyk, Józefa, et al.
Publicado: (2023)

Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR
por: Feng, Junxia, et al.
Publicado: (2023)

Inhibition of Rumen Protozoa by Specific Inhibitors of Lysozyme and Peptidases in vitro
por: Park, Tansol, et al.
Publicado: (2019)

Tissue Specificity in Social Context-Dependent lysozyme Expression in Bumblebees
por: Lattorff, H. Michael G.
Publicado: (2020)

Responses of Transgenic Melatonin-Enriched Goats on LPS Stimulation and the Proteogenomic Profiles of Their PBMCs
por: Yang, Minghui, et al.
Publicado: (2018)

Lysozyme-Induced Nephropathy
por: Santoriello, Dominick, et al.
Publicado: (2016)

Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacterium in the intestine and improves postnatal growth of chicken
por: Wang, Hai, et al.
Publicado: (2016)

Lysozyme Photochemistry as a Function of Temperature. The Protective Effect of Nanoparticles on Lysozyme Photostability
por: Oliveira Silva, Catarina, et al.
Publicado: (2015)

Tracing the Negative Results of Multiplex Real-Time PCR Assay for Diagnosis of Bacterial Pediatrics Meningitis
por: Karimi, Abdollah, et al.
Publicado: (2023)

Tongue Epithelium Cells from shRNA Mediated Transgenic Goat Show High Resistance to Foot and Mouth Disease Virus
por: Li, Wenting, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_emmfv5gl9lufm6hbft9ommdv55