Cargando…
Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking
Gastric ulceration is among the most serious humanpublic health problems. Olea europea L. cv. Arbequina is one of the numerous olive varieties which have scarcely been studied. The reported antioxidant and anti-inflammatory potential of the olive plant make it a potential prophylactic natural produc...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146603/ https://www.ncbi.nlm.nih.gov/pubmed/33922167 http://dx.doi.org/10.3390/antiox10050644 |
_version_ | 1783697433963790336 |
---|---|
author | Musa, Arafa Shady, Nourhan Hisham Ahmed, Shaimaa R. Alnusaire, Taghreed S. Sayed, Ahmed M. Alowaiesh, Bassam F. Sabouni, Ibrahim Al-Sanea, Mohammad M. Mostafa, Ehab M. Youssif, Khayrya A. Abu-Baih, Dalia H. Elrehany, Mahmoud A. Abdelmohsen, Usama Ramadan |
author_facet | Musa, Arafa Shady, Nourhan Hisham Ahmed, Shaimaa R. Alnusaire, Taghreed S. Sayed, Ahmed M. Alowaiesh, Bassam F. Sabouni, Ibrahim Al-Sanea, Mohammad M. Mostafa, Ehab M. Youssif, Khayrya A. Abu-Baih, Dalia H. Elrehany, Mahmoud A. Abdelmohsen, Usama Ramadan |
author_sort | Musa, Arafa |
collection | PubMed |
description | Gastric ulceration is among the most serious humanpublic health problems. Olea europea L. cv. Arbequina is one of the numerous olive varieties which have scarcely been studied. The reported antioxidant and anti-inflammatory potential of the olive plant make it a potential prophylactic natural product against gastric ulcers. Consequently, the main goal of this study is to investigate the gastroprotective effect of Olea europea L. cv. Arbequina leaf extract. LC-HRMS-based metabolic profiling of the alcoholic extract of Olea europea L. cv. Arbequina led to the dereplication of 18 putative compounds (1–18). In vivo indomethacin-induced gastric ulcer in a rat model was established and the Olea europea extract was tested at a dose of 300 mg kg(−1) compared to cimetidine (100 mg kg(−1)). The assessment of gastric mucosal lesions and histopathology of gastric tissue was done. It has been proved that Olea europea significantly decreased the ulcer index and protected the mucosa from lesions. The antioxidant potential of the extract was evaluated using three in vitro assays, H(2)O(2) scavenging, xanthine oxidase inhibitory, and superoxide radical scavenging activities and showed promising activities. Moreover, an in silico based study was performed on the putatively dereplicated compounds, which highlighted that 3-hydroxy tyrosol (4) and oleacein (18) can target the 5-lipoxygenase enzyme (5-LOX) as a protective mechanism against the pathogenesis of ulceration. Upon experimental validation, both compounds 3-hydroxy tyrosol (HT) and oleacein (OC) (4 and 18, respectively) exhibited a significant in vitro 5-LOX inhibitory activity with IC(50) values of 8.6 and 5.8 µg/mL, respectively. The present study suggested a possible implication of O. europea leaves as a potential candidate having gastroprotective, antioxidant, and 5-LOX inhibitory activity for the management of gastric ulcers. |
format | Online Article Text |
id | pubmed-8146603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81466032021-05-26 Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking Musa, Arafa Shady, Nourhan Hisham Ahmed, Shaimaa R. Alnusaire, Taghreed S. Sayed, Ahmed M. Alowaiesh, Bassam F. Sabouni, Ibrahim Al-Sanea, Mohammad M. Mostafa, Ehab M. Youssif, Khayrya A. Abu-Baih, Dalia H. Elrehany, Mahmoud A. Abdelmohsen, Usama Ramadan Antioxidants (Basel) Article Gastric ulceration is among the most serious humanpublic health problems. Olea europea L. cv. Arbequina is one of the numerous olive varieties which have scarcely been studied. The reported antioxidant and anti-inflammatory potential of the olive plant make it a potential prophylactic natural product against gastric ulcers. Consequently, the main goal of this study is to investigate the gastroprotective effect of Olea europea L. cv. Arbequina leaf extract. LC-HRMS-based metabolic profiling of the alcoholic extract of Olea europea L. cv. Arbequina led to the dereplication of 18 putative compounds (1–18). In vivo indomethacin-induced gastric ulcer in a rat model was established and the Olea europea extract was tested at a dose of 300 mg kg(−1) compared to cimetidine (100 mg kg(−1)). The assessment of gastric mucosal lesions and histopathology of gastric tissue was done. It has been proved that Olea europea significantly decreased the ulcer index and protected the mucosa from lesions. The antioxidant potential of the extract was evaluated using three in vitro assays, H(2)O(2) scavenging, xanthine oxidase inhibitory, and superoxide radical scavenging activities and showed promising activities. Moreover, an in silico based study was performed on the putatively dereplicated compounds, which highlighted that 3-hydroxy tyrosol (4) and oleacein (18) can target the 5-lipoxygenase enzyme (5-LOX) as a protective mechanism against the pathogenesis of ulceration. Upon experimental validation, both compounds 3-hydroxy tyrosol (HT) and oleacein (OC) (4 and 18, respectively) exhibited a significant in vitro 5-LOX inhibitory activity with IC(50) values of 8.6 and 5.8 µg/mL, respectively. The present study suggested a possible implication of O. europea leaves as a potential candidate having gastroprotective, antioxidant, and 5-LOX inhibitory activity for the management of gastric ulcers. MDPI 2021-04-22 /pmc/articles/PMC8146603/ /pubmed/33922167 http://dx.doi.org/10.3390/antiox10050644 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Musa, Arafa Shady, Nourhan Hisham Ahmed, Shaimaa R. Alnusaire, Taghreed S. Sayed, Ahmed M. Alowaiesh, Bassam F. Sabouni, Ibrahim Al-Sanea, Mohammad M. Mostafa, Ehab M. Youssif, Khayrya A. Abu-Baih, Dalia H. Elrehany, Mahmoud A. Abdelmohsen, Usama Ramadan Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking |
title | Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking |
title_full | Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking |
title_fullStr | Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking |
title_full_unstemmed | Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking |
title_short | Antiulcer Potential of Olea europea L. cv. Arbequina Leaf Extract Supported by Metabolic Profiling and Molecular Docking |
title_sort | antiulcer potential of olea europea l. cv. arbequina leaf extract supported by metabolic profiling and molecular docking |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146603/ https://www.ncbi.nlm.nih.gov/pubmed/33922167 http://dx.doi.org/10.3390/antiox10050644 |
work_keys_str_mv | AT musaarafa antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT shadynourhanhisham antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT ahmedshaimaar antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT alnusairetaghreeds antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT sayedahmedm antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT alowaieshbassamf antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT sabouniibrahim antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT alsaneamohammadm antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT mostafaehabm antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT youssifkhayryaa antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT abubaihdaliah antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT elrehanymahmouda antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking AT abdelmohsenusamaramadan antiulcerpotentialofoleaeuropealcvarbequinaleafextractsupportedbymetabolicprofilingandmoleculardocking |