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A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous...

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Autores principales: Liu, Xuemei, Guo, Weiling, Cui, Shumao, Tang, Xin, Zhao, Jianxin, Zhang, Hao, Mao, Bingyong, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146736/
https://www.ncbi.nlm.nih.gov/pubmed/33922843
http://dx.doi.org/10.3390/microorganisms9050908
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author Liu, Xuemei
Guo, Weiling
Cui, Shumao
Tang, Xin
Zhao, Jianxin
Zhang, Hao
Mao, Bingyong
Chen, Wei
author_facet Liu, Xuemei
Guo, Weiling
Cui, Shumao
Tang, Xin
Zhao, Jianxin
Zhang, Hao
Mao, Bingyong
Chen, Wei
author_sort Liu, Xuemei
collection PubMed
description In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.
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spelling pubmed-81467362021-05-26 A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 Liu, Xuemei Guo, Weiling Cui, Shumao Tang, Xin Zhao, Jianxin Zhang, Hao Mao, Bingyong Chen, Wei Microorganisms Article In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations. MDPI 2021-04-23 /pmc/articles/PMC8146736/ /pubmed/33922843 http://dx.doi.org/10.3390/microorganisms9050908 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xuemei
Guo, Weiling
Cui, Shumao
Tang, Xin
Zhao, Jianxin
Zhang, Hao
Mao, Bingyong
Chen, Wei
A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
title A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
title_full A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
title_fullStr A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
title_full_unstemmed A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
title_short A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
title_sort comprehensive assessment of the safety of blautia producta dsm 2950
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146736/
https://www.ncbi.nlm.nih.gov/pubmed/33922843
http://dx.doi.org/10.3390/microorganisms9050908
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