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A Comprehensive Assessment of the Safety of Blautia producta DSM 2950
In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146736/ https://www.ncbi.nlm.nih.gov/pubmed/33922843 http://dx.doi.org/10.3390/microorganisms9050908 |
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author | Liu, Xuemei Guo, Weiling Cui, Shumao Tang, Xin Zhao, Jianxin Zhang, Hao Mao, Bingyong Chen, Wei |
author_facet | Liu, Xuemei Guo, Weiling Cui, Shumao Tang, Xin Zhao, Jianxin Zhang, Hao Mao, Bingyong Chen, Wei |
author_sort | Liu, Xuemei |
collection | PubMed |
description | In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations. |
format | Online Article Text |
id | pubmed-8146736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81467362021-05-26 A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 Liu, Xuemei Guo, Weiling Cui, Shumao Tang, Xin Zhao, Jianxin Zhang, Hao Mao, Bingyong Chen, Wei Microorganisms Article In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations. MDPI 2021-04-23 /pmc/articles/PMC8146736/ /pubmed/33922843 http://dx.doi.org/10.3390/microorganisms9050908 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Xuemei Guo, Weiling Cui, Shumao Tang, Xin Zhao, Jianxin Zhang, Hao Mao, Bingyong Chen, Wei A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 |
title | A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 |
title_full | A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 |
title_fullStr | A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 |
title_full_unstemmed | A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 |
title_short | A Comprehensive Assessment of the Safety of Blautia producta DSM 2950 |
title_sort | comprehensive assessment of the safety of blautia producta dsm 2950 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146736/ https://www.ncbi.nlm.nih.gov/pubmed/33922843 http://dx.doi.org/10.3390/microorganisms9050908 |
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