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miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells
(1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been im...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146800/ https://www.ncbi.nlm.nih.gov/pubmed/33922649 http://dx.doi.org/10.3390/genes12050633 |
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author | Kim, Junhyung Park, Min Woo Park, Young Joon Ahn, Ju Won Sim, Jeong Min Kim, Suwan Heo, Jinhyung Jeong, Ji Hun Lee, Mihye Lim, Jaejoon Moon, Jong-Seok |
author_facet | Kim, Junhyung Park, Min Woo Park, Young Joon Ahn, Ju Won Sim, Jeong Min Kim, Suwan Heo, Jinhyung Jeong, Ji Hun Lee, Mihye Lim, Jaejoon Moon, Jong-Seok |
author_sort | Kim, Junhyung |
collection | PubMed |
description | (1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been implicated in several tumors including gliomas. However, the role of miR-542-3p in glucose metabolism in human gliomas remains unclear; (2) Methods: We measured the levels of cellular proliferation in human glioma cells. We measured the glycolytic activity in miR-542-3p knockdown and over-expressed human glioma cells. We measured the levels of miR-542-3p and HK2 in glioma tissues from patients with low- and high-grade gliomas using imaging analysis; (3) Results: We show that knockdown of miR-542-3p significantly suppressed cellular proliferation in human glioma cells. Knockdown of miR-542-3p suppressed HK2-induced glycolytic activity in human glioma cells. Consistently, over-expression of miR-542-3p increased HK2-induced glycolytic activity in human glioma cells. The levels of miR-542-3p and HK2 were significantly elevated in glioma tissues of patients with high-grade gliomas relative to that in low-grade gliomas. The elevation of HK2 levels in patients with high-grade gliomas were positively correlated with the high levels of miR-542-3p in GBM and low-grade gliomas (LGG) based on the datasets from the Cancer Genome Atlas (TCGA) database. Moreover, the high levels of miR-542-3p were associated with poor survival rate in the TCGA database; (4) Conclusions: miR-542-3p contributes to the HK2-mediated high glycolytic phenotype in human glioma cells. |
format | Online Article Text |
id | pubmed-8146800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81468002021-05-26 miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells Kim, Junhyung Park, Min Woo Park, Young Joon Ahn, Ju Won Sim, Jeong Min Kim, Suwan Heo, Jinhyung Jeong, Ji Hun Lee, Mihye Lim, Jaejoon Moon, Jong-Seok Genes (Basel) Article (1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been implicated in several tumors including gliomas. However, the role of miR-542-3p in glucose metabolism in human gliomas remains unclear; (2) Methods: We measured the levels of cellular proliferation in human glioma cells. We measured the glycolytic activity in miR-542-3p knockdown and over-expressed human glioma cells. We measured the levels of miR-542-3p and HK2 in glioma tissues from patients with low- and high-grade gliomas using imaging analysis; (3) Results: We show that knockdown of miR-542-3p significantly suppressed cellular proliferation in human glioma cells. Knockdown of miR-542-3p suppressed HK2-induced glycolytic activity in human glioma cells. Consistently, over-expression of miR-542-3p increased HK2-induced glycolytic activity in human glioma cells. The levels of miR-542-3p and HK2 were significantly elevated in glioma tissues of patients with high-grade gliomas relative to that in low-grade gliomas. The elevation of HK2 levels in patients with high-grade gliomas were positively correlated with the high levels of miR-542-3p in GBM and low-grade gliomas (LGG) based on the datasets from the Cancer Genome Atlas (TCGA) database. Moreover, the high levels of miR-542-3p were associated with poor survival rate in the TCGA database; (4) Conclusions: miR-542-3p contributes to the HK2-mediated high glycolytic phenotype in human glioma cells. MDPI 2021-04-23 /pmc/articles/PMC8146800/ /pubmed/33922649 http://dx.doi.org/10.3390/genes12050633 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Junhyung Park, Min Woo Park, Young Joon Ahn, Ju Won Sim, Jeong Min Kim, Suwan Heo, Jinhyung Jeong, Ji Hun Lee, Mihye Lim, Jaejoon Moon, Jong-Seok miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
title | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
title_full | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
title_fullStr | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
title_full_unstemmed | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
title_short | miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cells |
title_sort | mir-542-3p contributes to the hk2-mediated high glycolytic phenotype in human glioma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146800/ https://www.ncbi.nlm.nih.gov/pubmed/33922649 http://dx.doi.org/10.3390/genes12050633 |
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