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Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study
BACKGROUND: Atherosclerosis is the main etiology of cardiovascular diseases (CVD), associated to systemic inflammation. Matrix metalloproteinases (MMPs) are related to atherosclerosis progression through the SDF1/CXCR4 axis promoting macrophages recruitment within the vascular wall. The goal was to...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147178/ https://www.ncbi.nlm.nih.gov/pubmed/34062730 http://dx.doi.org/10.3390/life11050414 |
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author | Marcos-Jubilar, María Orbe, Josune Roncal, Carmen Machado, Florencio J. D. Rodriguez, José Antonio Fernández-Montero, Alejandro Colina, Inmaculada Rodil, Raquel Pastrana, Juan C. Páramo, José A. |
author_facet | Marcos-Jubilar, María Orbe, Josune Roncal, Carmen Machado, Florencio J. D. Rodriguez, José Antonio Fernández-Montero, Alejandro Colina, Inmaculada Rodil, Raquel Pastrana, Juan C. Páramo, José A. |
author_sort | Marcos-Jubilar, María |
collection | PubMed |
description | BACKGROUND: Atherosclerosis is the main etiology of cardiovascular diseases (CVD), associated to systemic inflammation. Matrix metalloproteinases (MMPs) are related to atherosclerosis progression through the SDF1/CXCR4 axis promoting macrophages recruitment within the vascular wall. The goal was to assess new circulatory inflammatory markers in relation to atherosclerosis. METHODS: Measurement of SDF1, MMP12 and CRP in blood samples of 298 prospective patients with cardiovascular risk. To explore atherosclerosis progression, CXCR4/SDF1 axis and MMP12 expression were determined by RT-qPCR and by immunohistochemistry in the aorta of accelerated and delayed atherosclerosis mice models (Apoe-/- and Apoe-/-Mmp10-/-). RESULTS: SDF1, MMP12 and CRP were elevated in patients with clinical atherosclerosis, but after controlling by confounding factors, only SDF1 and CRP remained increased. Having high levels of both biomarkers showed 2.8-fold increased risk of presenting clinical atherosclerosis (p = 0.022). Patients with elevated SDF1, MMP12 and CRP showed increased risk of death in follow-up (HR = 3.2, 95%CI: 1.5–7.0, p = 0.004). Gene and protein expression of CXCR4 and MMP12 were increased in aortas from Apoe-/- mice. CONCLUSIONS: The combination of high circulating SDF1, MMP12 and CRP identified patients with particular inflammatory cardiovascular risk and increased mortality. SDF1/CXCR4 axis and MMP12 involvement in atherosclerosis development suggests that they could be possible atherosclerotic targets. |
format | Online Article Text |
id | pubmed-8147178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81471782021-05-26 Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study Marcos-Jubilar, María Orbe, Josune Roncal, Carmen Machado, Florencio J. D. Rodriguez, José Antonio Fernández-Montero, Alejandro Colina, Inmaculada Rodil, Raquel Pastrana, Juan C. Páramo, José A. Life (Basel) Article BACKGROUND: Atherosclerosis is the main etiology of cardiovascular diseases (CVD), associated to systemic inflammation. Matrix metalloproteinases (MMPs) are related to atherosclerosis progression through the SDF1/CXCR4 axis promoting macrophages recruitment within the vascular wall. The goal was to assess new circulatory inflammatory markers in relation to atherosclerosis. METHODS: Measurement of SDF1, MMP12 and CRP in blood samples of 298 prospective patients with cardiovascular risk. To explore atherosclerosis progression, CXCR4/SDF1 axis and MMP12 expression were determined by RT-qPCR and by immunohistochemistry in the aorta of accelerated and delayed atherosclerosis mice models (Apoe-/- and Apoe-/-Mmp10-/-). RESULTS: SDF1, MMP12 and CRP were elevated in patients with clinical atherosclerosis, but after controlling by confounding factors, only SDF1 and CRP remained increased. Having high levels of both biomarkers showed 2.8-fold increased risk of presenting clinical atherosclerosis (p = 0.022). Patients with elevated SDF1, MMP12 and CRP showed increased risk of death in follow-up (HR = 3.2, 95%CI: 1.5–7.0, p = 0.004). Gene and protein expression of CXCR4 and MMP12 were increased in aortas from Apoe-/- mice. CONCLUSIONS: The combination of high circulating SDF1, MMP12 and CRP identified patients with particular inflammatory cardiovascular risk and increased mortality. SDF1/CXCR4 axis and MMP12 involvement in atherosclerosis development suggests that they could be possible atherosclerotic targets. MDPI 2021-05-01 /pmc/articles/PMC8147178/ /pubmed/34062730 http://dx.doi.org/10.3390/life11050414 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marcos-Jubilar, María Orbe, Josune Roncal, Carmen Machado, Florencio J. D. Rodriguez, José Antonio Fernández-Montero, Alejandro Colina, Inmaculada Rodil, Raquel Pastrana, Juan C. Páramo, José A. Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study |
title | Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study |
title_full | Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study |
title_fullStr | Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study |
title_full_unstemmed | Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study |
title_short | Association of SDF1 and MMP12 with Atherosclerosis and Inflammation: Clinical and Experimental Study |
title_sort | association of sdf1 and mmp12 with atherosclerosis and inflammation: clinical and experimental study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147178/ https://www.ncbi.nlm.nih.gov/pubmed/34062730 http://dx.doi.org/10.3390/life11050414 |
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