Cargando…

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

SIMPLE SUMMARY: Fipronil (FPN) is an insecticide that can be used in insect control in various cereal crops in agriculture, veterinary activities, and public health management. Boswellic acid (BA) is a pentacyclic triterpene, which is a compound isolated from Boswellia serrata gum resin. This study...

Descripción completa

Detalles Bibliográficos
Autores principales: Tohamy, Hossam G., El-Kazaz, Sara E., Alotaibi, Saqer S., Ibrahiem, Hawary S., Shukry, Mustafa, Dawood, Mahmoud A. O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147226/
https://www.ncbi.nlm.nih.gov/pubmed/33946602
http://dx.doi.org/10.3390/ani11051302
_version_ 1783697581082148864
author Tohamy, Hossam G.
El-Kazaz, Sara E.
Alotaibi, Saqer S.
Ibrahiem, Hawary S.
Shukry, Mustafa
Dawood, Mahmoud A. O.
author_facet Tohamy, Hossam G.
El-Kazaz, Sara E.
Alotaibi, Saqer S.
Ibrahiem, Hawary S.
Shukry, Mustafa
Dawood, Mahmoud A. O.
author_sort Tohamy, Hossam G.
collection PubMed
description SIMPLE SUMMARY: Fipronil (FPN) is an insecticide that can be used in insect control in various cereal crops in agriculture, veterinary activities, and public health management. Boswellic acid (BA) is a pentacyclic triterpene, which is a compound isolated from Boswellia serrata gum resin. This study was designed to determine BA’s potential protective impact against oxidative and testicular damage caused by FPN insecticide poisoning on the male rat model. BA significantly improved the reproductive parameters assessed, such as the number of pregnant females, index of pregnancy and the number of litters, weights of the reproductive organ, sperm cell quality, morphological alterations of testes, epididymis, and sex glands by accessory caused by FPN oxidative stress, as well as the improvement of steroidogenesis, antioxidants, and antiapoptotic marker. ABSTRACT: The study investigated the ability of boswellic acid (BA) to alleviate the testicular and oxidative injury FPN insecticide intoxication in the male rat model. Rats were randomly assigned to six equivalent groups (six rats each) as the following: control rats orally administered with 2 mL physiological saline/kg of body weight (bwt); boswellic acid (BA1) rats orally administered 250 mg BA/kg bwt; boswellic acid (BA2) rats orally administered 500 mg BA/kg bwt; fipronil (FPN) rats orally administered 20 mg FPN/kg bwt; (FPN + BA1) rats orally administered 20 mg FPN/kg bwt plus 250 mg BA/kg bwt, and (FPN + BA2) rats orally administered 20 mg FPN/kg bwt plus 500 mg BA/kg bwt. After 60 days, semen viability percentage and live spermatozoa percentage were decreased, and a considerably increased abnormality of the sperm cells in FPN-administered rats improved substantially with the co-administration of BA. BA had refinement of the histological architecture of testes and sexual glands. Quantitative analysis recorded a noticeable decline in the nuclear cell-proliferating antigen (PCNA) percentage area. FPN triggered cell damage, which was suggested by elevated malondialdehyde and interleukin 6, tumor necrosis factors alpha, and decreased glutathione level. Proapoptotic factor overexpression is mediated by FPN administration, while it decreased the antiapoptotic protein expression. Similarly, BA has shown significant upregulation in steroidogenic and fertility-related gene expression concerning the FPN group. Pathophysiological damages induced by FPN could be alleviated by BA’s antioxidant ability and antiapoptotic factor alongside the upregulation of steroidogenic and fertility-related genes and regimented the detrimental effects of FPN on antioxidant and pro-inflammatory biomarkers.
format Online
Article
Text
id pubmed-8147226
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81472262021-05-26 Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats Tohamy, Hossam G. El-Kazaz, Sara E. Alotaibi, Saqer S. Ibrahiem, Hawary S. Shukry, Mustafa Dawood, Mahmoud A. O. Animals (Basel) Article SIMPLE SUMMARY: Fipronil (FPN) is an insecticide that can be used in insect control in various cereal crops in agriculture, veterinary activities, and public health management. Boswellic acid (BA) is a pentacyclic triterpene, which is a compound isolated from Boswellia serrata gum resin. This study was designed to determine BA’s potential protective impact against oxidative and testicular damage caused by FPN insecticide poisoning on the male rat model. BA significantly improved the reproductive parameters assessed, such as the number of pregnant females, index of pregnancy and the number of litters, weights of the reproductive organ, sperm cell quality, morphological alterations of testes, epididymis, and sex glands by accessory caused by FPN oxidative stress, as well as the improvement of steroidogenesis, antioxidants, and antiapoptotic marker. ABSTRACT: The study investigated the ability of boswellic acid (BA) to alleviate the testicular and oxidative injury FPN insecticide intoxication in the male rat model. Rats were randomly assigned to six equivalent groups (six rats each) as the following: control rats orally administered with 2 mL physiological saline/kg of body weight (bwt); boswellic acid (BA1) rats orally administered 250 mg BA/kg bwt; boswellic acid (BA2) rats orally administered 500 mg BA/kg bwt; fipronil (FPN) rats orally administered 20 mg FPN/kg bwt; (FPN + BA1) rats orally administered 20 mg FPN/kg bwt plus 250 mg BA/kg bwt, and (FPN + BA2) rats orally administered 20 mg FPN/kg bwt plus 500 mg BA/kg bwt. After 60 days, semen viability percentage and live spermatozoa percentage were decreased, and a considerably increased abnormality of the sperm cells in FPN-administered rats improved substantially with the co-administration of BA. BA had refinement of the histological architecture of testes and sexual glands. Quantitative analysis recorded a noticeable decline in the nuclear cell-proliferating antigen (PCNA) percentage area. FPN triggered cell damage, which was suggested by elevated malondialdehyde and interleukin 6, tumor necrosis factors alpha, and decreased glutathione level. Proapoptotic factor overexpression is mediated by FPN administration, while it decreased the antiapoptotic protein expression. Similarly, BA has shown significant upregulation in steroidogenic and fertility-related gene expression concerning the FPN group. Pathophysiological damages induced by FPN could be alleviated by BA’s antioxidant ability and antiapoptotic factor alongside the upregulation of steroidogenic and fertility-related genes and regimented the detrimental effects of FPN on antioxidant and pro-inflammatory biomarkers. MDPI 2021-04-30 /pmc/articles/PMC8147226/ /pubmed/33946602 http://dx.doi.org/10.3390/ani11051302 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tohamy, Hossam G.
El-Kazaz, Sara E.
Alotaibi, Saqer S.
Ibrahiem, Hawary S.
Shukry, Mustafa
Dawood, Mahmoud A. O.
Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats
title Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats
title_full Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats
title_fullStr Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats
title_full_unstemmed Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats
title_short Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats
title_sort ameliorative effects of boswellic acid on fipronil-induced toxicity: antioxidant state, apoptotic markers, and testicular steroidogenic expression in male rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147226/
https://www.ncbi.nlm.nih.gov/pubmed/33946602
http://dx.doi.org/10.3390/ani11051302
work_keys_str_mv AT tohamyhossamg ameliorativeeffectsofboswellicacidonfipronilinducedtoxicityantioxidantstateapoptoticmarkersandtesticularsteroidogenicexpressioninmalerats
AT elkazazsarae ameliorativeeffectsofboswellicacidonfipronilinducedtoxicityantioxidantstateapoptoticmarkersandtesticularsteroidogenicexpressioninmalerats
AT alotaibisaqers ameliorativeeffectsofboswellicacidonfipronilinducedtoxicityantioxidantstateapoptoticmarkersandtesticularsteroidogenicexpressioninmalerats
AT ibrahiemhawarys ameliorativeeffectsofboswellicacidonfipronilinducedtoxicityantioxidantstateapoptoticmarkersandtesticularsteroidogenicexpressioninmalerats
AT shukrymustafa ameliorativeeffectsofboswellicacidonfipronilinducedtoxicityantioxidantstateapoptoticmarkersandtesticularsteroidogenicexpressioninmalerats
AT dawoodmahmoudao ameliorativeeffectsofboswellicacidonfipronilinducedtoxicityantioxidantstateapoptoticmarkersandtesticularsteroidogenicexpressioninmalerats