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A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs

Circulating miRNAs are ideal diagnostics and prognostics biomarkers in cancer since altered levels of specific miRNAs have been associated to development/progression of several cancers. Physical activity is a recognized preventive strategy against several cancers, but it may also modify the baseline...

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Autores principales: Faraldi, Martina, Gerosa, Laura, Gomarasca, Marta, Sansoni, Veronica, Perego, Silvia, Ziemann, Ewa, Banfi, Giuseppe, Lombardi, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147229/
https://www.ncbi.nlm.nih.gov/pubmed/33946605
http://dx.doi.org/10.3390/diagnostics11050820
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author Faraldi, Martina
Gerosa, Laura
Gomarasca, Marta
Sansoni, Veronica
Perego, Silvia
Ziemann, Ewa
Banfi, Giuseppe
Lombardi, Giovanni
author_facet Faraldi, Martina
Gerosa, Laura
Gomarasca, Marta
Sansoni, Veronica
Perego, Silvia
Ziemann, Ewa
Banfi, Giuseppe
Lombardi, Giovanni
author_sort Faraldi, Martina
collection PubMed
description Circulating miRNAs are ideal diagnostics and prognostics biomarkers in cancer since altered levels of specific miRNAs have been associated to development/progression of several cancers. Physical activity is a recognized preventive strategy against several cancers, but it may also modify the baseline levels of cancer-associated miRNAs and, hence, may act as a confounding pre-analytical variable. This study aimed at understanding whether physical activity-dependent changes in cancer-associated circulating miRNAs profile could act as a confounding variable. A panel comprising 179 miRNAs was assayed in plasma from 20 highly trained and 10 sedentary men. RT-qPCR data were analyzed with the 2(−2ΔΔCT) methods and normalized on hsa-miR-320d, as determined by bioinformatics analysis. miRNAs associated with the diagnosis of the most prevalent cancers were considered. Only those miRNAs, relevantly associated with cancers, found ≥2-fold up- or downregulated in highly trained subjects compared to sedentary were disclosed. The results reveal that chronic physical activity determined modifications altering the baseline level of several cancer-associated miRNAs and, hence, their diagnostic and prognostic potential. In conclusion, based on our results, a physically active status emerges as an important pre-analytical variable able to alter the basal level of circulating miRNAs, and these alterations might be considered as potentially misleading the analytical output.
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spelling pubmed-81472292021-05-26 A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs Faraldi, Martina Gerosa, Laura Gomarasca, Marta Sansoni, Veronica Perego, Silvia Ziemann, Ewa Banfi, Giuseppe Lombardi, Giovanni Diagnostics (Basel) Article Circulating miRNAs are ideal diagnostics and prognostics biomarkers in cancer since altered levels of specific miRNAs have been associated to development/progression of several cancers. Physical activity is a recognized preventive strategy against several cancers, but it may also modify the baseline levels of cancer-associated miRNAs and, hence, may act as a confounding pre-analytical variable. This study aimed at understanding whether physical activity-dependent changes in cancer-associated circulating miRNAs profile could act as a confounding variable. A panel comprising 179 miRNAs was assayed in plasma from 20 highly trained and 10 sedentary men. RT-qPCR data were analyzed with the 2(−2ΔΔCT) methods and normalized on hsa-miR-320d, as determined by bioinformatics analysis. miRNAs associated with the diagnosis of the most prevalent cancers were considered. Only those miRNAs, relevantly associated with cancers, found ≥2-fold up- or downregulated in highly trained subjects compared to sedentary were disclosed. The results reveal that chronic physical activity determined modifications altering the baseline level of several cancer-associated miRNAs and, hence, their diagnostic and prognostic potential. In conclusion, based on our results, a physically active status emerges as an important pre-analytical variable able to alter the basal level of circulating miRNAs, and these alterations might be considered as potentially misleading the analytical output. MDPI 2021-04-30 /pmc/articles/PMC8147229/ /pubmed/33946605 http://dx.doi.org/10.3390/diagnostics11050820 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Faraldi, Martina
Gerosa, Laura
Gomarasca, Marta
Sansoni, Veronica
Perego, Silvia
Ziemann, Ewa
Banfi, Giuseppe
Lombardi, Giovanni
A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs
title A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs
title_full A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs
title_fullStr A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs
title_full_unstemmed A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs
title_short A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs
title_sort physically active status affects the circulating profile of cancer-associated mirnas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147229/
https://www.ncbi.nlm.nih.gov/pubmed/33946605
http://dx.doi.org/10.3390/diagnostics11050820
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