Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels

A protocol based on the combination of different analytical methodologies is proposed to standardize the experimental conditions for reproducible formulations of hybrid hydrogels. The final hybrid material, based on the combination of gelatin and chitosan functionalized with methylfuran and cross-li...

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Detalles Bibliográficos
Autores principales: Magli, Sofia, Rossi, Lorenzo, Consentino, Cesare, Bertini, Sabrina, Nicotra, Francesco, Russo, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147276/
https://www.ncbi.nlm.nih.gov/pubmed/34062918
http://dx.doi.org/10.3390/biom11050683
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author Magli, Sofia
Rossi, Lorenzo
Consentino, Cesare
Bertini, Sabrina
Nicotra, Francesco
Russo, Laura
author_facet Magli, Sofia
Rossi, Lorenzo
Consentino, Cesare
Bertini, Sabrina
Nicotra, Francesco
Russo, Laura
author_sort Magli, Sofia
collection PubMed
description A protocol based on the combination of different analytical methodologies is proposed to standardize the experimental conditions for reproducible formulations of hybrid hydrogels. The final hybrid material, based on the combination of gelatin and chitosan functionalized with methylfuran and cross-linked with 4-arm-PEG-maleimide, is able to mimic role, dynamism, and structural complexity of the extracellular matrix. Physical–chemical properties of starting polymers and finals constructs were characterized exploiting the combination of HP-SEC-TDA, UV, FT-IR, NMR, and TGA.
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spelling pubmed-81472762021-05-26 Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels Magli, Sofia Rossi, Lorenzo Consentino, Cesare Bertini, Sabrina Nicotra, Francesco Russo, Laura Biomolecules Article A protocol based on the combination of different analytical methodologies is proposed to standardize the experimental conditions for reproducible formulations of hybrid hydrogels. The final hybrid material, based on the combination of gelatin and chitosan functionalized with methylfuran and cross-linked with 4-arm-PEG-maleimide, is able to mimic role, dynamism, and structural complexity of the extracellular matrix. Physical–chemical properties of starting polymers and finals constructs were characterized exploiting the combination of HP-SEC-TDA, UV, FT-IR, NMR, and TGA. MDPI 2021-05-01 /pmc/articles/PMC8147276/ /pubmed/34062918 http://dx.doi.org/10.3390/biom11050683 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Magli, Sofia
Rossi, Lorenzo
Consentino, Cesare
Bertini, Sabrina
Nicotra, Francesco
Russo, Laura
Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
title Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
title_full Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
title_fullStr Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
title_full_unstemmed Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
title_short Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
title_sort combined analytical approaches to standardize and characterize biomaterials formulations: application to chitosan-gelatin cross-linked hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147276/
https://www.ncbi.nlm.nih.gov/pubmed/34062918
http://dx.doi.org/10.3390/biom11050683
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