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Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM

Two colorimetric broth microdilution antifungal susceptibility tests were compared, Sensititre YeastOne and MICRONAUT-AM for nine antifungal agents. One hundred clinical Candida isolates were tested, representing a realistic population for susceptibility testing in daily practice. The reproducibilit...

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Autores principales: Philips, Sophie, Van Hoecke, Frederik, De Laere, Emmanuel, Vervaeke, Steven, De Smedt, Roos, Boelens, Jerina, De Geyter, Deborah, Piérard, Denis, Lagrou, Katrien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147297/
https://www.ncbi.nlm.nih.gov/pubmed/34062848
http://dx.doi.org/10.3390/jof7050356
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author Philips, Sophie
Van Hoecke, Frederik
De Laere, Emmanuel
Vervaeke, Steven
De Smedt, Roos
Boelens, Jerina
De Geyter, Deborah
Piérard, Denis
Lagrou, Katrien
author_facet Philips, Sophie
Van Hoecke, Frederik
De Laere, Emmanuel
Vervaeke, Steven
De Smedt, Roos
Boelens, Jerina
De Geyter, Deborah
Piérard, Denis
Lagrou, Katrien
author_sort Philips, Sophie
collection PubMed
description Two colorimetric broth microdilution antifungal susceptibility tests were compared, Sensititre YeastOne and MICRONAUT-AM for nine antifungal agents. One hundred clinical Candida isolates were tested, representing a realistic population for susceptibility testing in daily practice. The reproducibility characteristics were comparable. Only for fluconazole, caspofungin, 5-flucytosine and amphotericin B, an essential agreement of ≥90% could be demonstrated. Sensititre minimal inhibitory concentrations (MICs) were systematically higher than MICRONAUT MICs for all antifungals, except for itraconazole. CLSI clinical breakpoints (CBPs) and epidemiological cut-off values (ECVs) were used for Sensititre MICs while for MICRONAUT the EUCAST CBPs and ECVs were used. Only fluconazole, micafungin, and amphotericin B had a categorical agreement of ≥90%. For fluconazole, micafungin, and amphotericin B the susceptibility proportions were comparable. Susceptibility proportion of posaconazole and voriconazole was higher using the MICRONAUT system. For itraconazole and anidulafungin, the susceptibility proportion was higher using Sensititre. It was not possible to determine the true MIC values or the correctness of a S/I/R result since both commercial systems were validated against a different reference method. These findings show that there is a significant variability in susceptibility pattern and consequently on use of antifungals in daily practice, depending on the choice of commercial system.
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spelling pubmed-81472972021-05-26 Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM Philips, Sophie Van Hoecke, Frederik De Laere, Emmanuel Vervaeke, Steven De Smedt, Roos Boelens, Jerina De Geyter, Deborah Piérard, Denis Lagrou, Katrien J Fungi (Basel) Article Two colorimetric broth microdilution antifungal susceptibility tests were compared, Sensititre YeastOne and MICRONAUT-AM for nine antifungal agents. One hundred clinical Candida isolates were tested, representing a realistic population for susceptibility testing in daily practice. The reproducibility characteristics were comparable. Only for fluconazole, caspofungin, 5-flucytosine and amphotericin B, an essential agreement of ≥90% could be demonstrated. Sensititre minimal inhibitory concentrations (MICs) were systematically higher than MICRONAUT MICs for all antifungals, except for itraconazole. CLSI clinical breakpoints (CBPs) and epidemiological cut-off values (ECVs) were used for Sensititre MICs while for MICRONAUT the EUCAST CBPs and ECVs were used. Only fluconazole, micafungin, and amphotericin B had a categorical agreement of ≥90%. For fluconazole, micafungin, and amphotericin B the susceptibility proportions were comparable. Susceptibility proportion of posaconazole and voriconazole was higher using the MICRONAUT system. For itraconazole and anidulafungin, the susceptibility proportion was higher using Sensititre. It was not possible to determine the true MIC values or the correctness of a S/I/R result since both commercial systems were validated against a different reference method. These findings show that there is a significant variability in susceptibility pattern and consequently on use of antifungals in daily practice, depending on the choice of commercial system. MDPI 2021-05-01 /pmc/articles/PMC8147297/ /pubmed/34062848 http://dx.doi.org/10.3390/jof7050356 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Philips, Sophie
Van Hoecke, Frederik
De Laere, Emmanuel
Vervaeke, Steven
De Smedt, Roos
Boelens, Jerina
De Geyter, Deborah
Piérard, Denis
Lagrou, Katrien
Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM
title Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM
title_full Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM
title_fullStr Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM
title_full_unstemmed Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM
title_short Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM
title_sort comparison of two commercial colorimetric broth microdilution tests for candida susceptibility testing: sensititre yeastone versus micronaut-am
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147297/
https://www.ncbi.nlm.nih.gov/pubmed/34062848
http://dx.doi.org/10.3390/jof7050356
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