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Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration
Poly(ADP-ribose) polymerase-1 (Parp-1) is involved in DNA repair and cell-death induction after DNA damage. Parp-1(−/−) mice show higher susceptibility to the carcinogenic effects of nitrosamine and azoxymethane. To elucidate the role of alterations of the PARP-1 gene in human carcinogenesis, we exa...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japan Academy
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147301/ |
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author | Masutani, Mitsuko Nozaki, Tadashige Sasaki, Hiroki Yamada, Tesshi Kohno, Takashi Shimizu, Kimiko Gotoh, Masahiro Shiraishi, Masahiko Yokota, Jun Hirohashi, Setsuo Nakagama, Hitoshi Sugimura, Takashi |
author_facet | Masutani, Mitsuko Nozaki, Tadashige Sasaki, Hiroki Yamada, Tesshi Kohno, Takashi Shimizu, Kimiko Gotoh, Masahiro Shiraishi, Masahiko Yokota, Jun Hirohashi, Setsuo Nakagama, Hitoshi Sugimura, Takashi |
author_sort | Masutani, Mitsuko |
collection | PubMed |
description | Poly(ADP-ribose) polymerase-1 (Parp-1) is involved in DNA repair and cell-death induction after DNA damage. Parp-1(−/−) mice show higher susceptibility to the carcinogenic effects of nitrosamine and azoxymethane. To elucidate the role of alterations of the PARP-1 gene in human carcinogenesis, we examined the expression level of PARP-1 gene in various human tumor cell lines. The presence of gross rearrangement of PARP-1 gene in these cell lines was also examined by Southern blot hybridization analysis. The expression levels of PARP-1 gene in several cell lines, including T-cell leukemia cell lines (Molt-4 and CCRF-CEM), colon cancer cell line (WiDr), and gastric cancer cell lines (KATOIII, OKAJIMA, and MKN45) was substantially lower than in other cancer cell lines. Among the 85 analyzed cell lines, structural alteration of PARP-1 gene was detected in a gastric cancer cell line, MKN28. A low level of PARP-1 expression in human cancer could potentially influence cancer cell growth, differentiation and cancer development by affecting genomic instability, as well as the response of tumors to chemo- and radiotherapy. |
format | Online Article Text |
id | pubmed-8147301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Japan Academy |
record_format | MEDLINE/PubMed |
spelling | pubmed-81473012021-05-26 Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration Masutani, Mitsuko Nozaki, Tadashige Sasaki, Hiroki Yamada, Tesshi Kohno, Takashi Shimizu, Kimiko Gotoh, Masahiro Shiraishi, Masahiko Yokota, Jun Hirohashi, Setsuo Nakagama, Hitoshi Sugimura, Takashi Proc Jpn Acad Ser B Phys Biol Sci Articles Poly(ADP-ribose) polymerase-1 (Parp-1) is involved in DNA repair and cell-death induction after DNA damage. Parp-1(−/−) mice show higher susceptibility to the carcinogenic effects of nitrosamine and azoxymethane. To elucidate the role of alterations of the PARP-1 gene in human carcinogenesis, we examined the expression level of PARP-1 gene in various human tumor cell lines. The presence of gross rearrangement of PARP-1 gene in these cell lines was also examined by Southern blot hybridization analysis. The expression levels of PARP-1 gene in several cell lines, including T-cell leukemia cell lines (Molt-4 and CCRF-CEM), colon cancer cell line (WiDr), and gastric cancer cell lines (KATOIII, OKAJIMA, and MKN45) was substantially lower than in other cancer cell lines. Among the 85 analyzed cell lines, structural alteration of PARP-1 gene was detected in a gastric cancer cell line, MKN28. A low level of PARP-1 expression in human cancer could potentially influence cancer cell growth, differentiation and cancer development by affecting genomic instability, as well as the response of tumors to chemo- and radiotherapy. The Japan Academy 2004-02 2004-02-01 /pmc/articles/PMC8147301/ Text en © 2004 The Japan Academy https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Masutani, Mitsuko Nozaki, Tadashige Sasaki, Hiroki Yamada, Tesshi Kohno, Takashi Shimizu, Kimiko Gotoh, Masahiro Shiraishi, Masahiko Yokota, Jun Hirohashi, Setsuo Nakagama, Hitoshi Sugimura, Takashi Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration |
title | Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration |
title_full | Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration |
title_fullStr | Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration |
title_full_unstemmed | Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration |
title_short | Poly(ADP-ribose) polymerase-1 gene in human tumor cell lines: Its expression and structural alteration |
title_sort | poly(adp-ribose) polymerase-1 gene in human tumor cell lines: its expression and structural alteration |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147301/ |
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