Cargando…
Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor
An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147326/ https://www.ncbi.nlm.nih.gov/pubmed/34062892 http://dx.doi.org/10.3390/cells10051084 |
_version_ | 1783697604641554432 |
---|---|
author | Ivanov, Ivan N. Zachleder, Vilém Vítová, Milada Barbosa, Maria J. Bišová, Kateřina |
author_facet | Ivanov, Ivan N. Zachleder, Vilém Vítová, Milada Barbosa, Maria J. Bišová, Kateřina |
author_sort | Ivanov, Ivan N. |
collection | PubMed |
description | An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39 °C) causes a complete block of nuclear and cellular division accompanied by an increased accumulation of starch. In this work we explore the potential of supraoptimal temperature as a method to promote starch production in C. reinhardtii in a pilot-scale photobioreactor. The method was successfully applied and resulted in an almost 3-fold increase in the starch content of C. reinhardtii dry matter. Moreover, a maximum starch content at the supraoptimal temperature was reached within 1–2 days, compared with 5 days for the control culture at the optimal temperature (30 °C). Therefore, supraoptimal temperature treatment promotes rapid starch accumulation and suggests a viable alternative to other starch-inducing methods, such as nutrient depletion. Nevertheless, technical challenges, such as bioreactor design and light availability within the culture, still need to be dealt with. |
format | Online Article Text |
id | pubmed-8147326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81473262021-05-26 Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor Ivanov, Ivan N. Zachleder, Vilém Vítová, Milada Barbosa, Maria J. Bišová, Kateřina Cells Article An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39 °C) causes a complete block of nuclear and cellular division accompanied by an increased accumulation of starch. In this work we explore the potential of supraoptimal temperature as a method to promote starch production in C. reinhardtii in a pilot-scale photobioreactor. The method was successfully applied and resulted in an almost 3-fold increase in the starch content of C. reinhardtii dry matter. Moreover, a maximum starch content at the supraoptimal temperature was reached within 1–2 days, compared with 5 days for the control culture at the optimal temperature (30 °C). Therefore, supraoptimal temperature treatment promotes rapid starch accumulation and suggests a viable alternative to other starch-inducing methods, such as nutrient depletion. Nevertheless, technical challenges, such as bioreactor design and light availability within the culture, still need to be dealt with. MDPI 2021-05-01 /pmc/articles/PMC8147326/ /pubmed/34062892 http://dx.doi.org/10.3390/cells10051084 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ivanov, Ivan N. Zachleder, Vilém Vítová, Milada Barbosa, Maria J. Bišová, Kateřina Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor |
title | Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor |
title_full | Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor |
title_fullStr | Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor |
title_full_unstemmed | Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor |
title_short | Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor |
title_sort | starch production in chlamydomonas reinhardtii through supraoptimal temperature in a pilot-scale photobioreactor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147326/ https://www.ncbi.nlm.nih.gov/pubmed/34062892 http://dx.doi.org/10.3390/cells10051084 |
work_keys_str_mv | AT ivanovivann starchproductioninchlamydomonasreinhardtiithroughsupraoptimaltemperatureinapilotscalephotobioreactor AT zachledervilem starchproductioninchlamydomonasreinhardtiithroughsupraoptimaltemperatureinapilotscalephotobioreactor AT vitovamilada starchproductioninchlamydomonasreinhardtiithroughsupraoptimaltemperatureinapilotscalephotobioreactor AT barbosamariaj starchproductioninchlamydomonasreinhardtiithroughsupraoptimaltemperatureinapilotscalephotobioreactor AT bisovakaterina starchproductioninchlamydomonasreinhardtiithroughsupraoptimaltemperatureinapilotscalephotobioreactor |