The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles

Wear debris generated from the bearing surfaces of joint arthroplasties leads to acute and chronic inflammation, which is strongly associated with implant failure. Macrophages derived from monocytes recruited to the local tissues have a significant impact on bone healing and regeneration. Macrophage...

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Autores principales: Gao, Qi, Rhee, Claire, Maruyama, Masahiro, Li, Zhong, Shen, Huaishuang, Zhang, Ning, Utsunomiya, Takeshi, Huang, Elijah Ejun, Yao, Zhenyu, Bunnell, Bruce A., Lin, Hang, Tuan, Rocky S., Goodman, Stuart B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147332/
https://www.ncbi.nlm.nih.gov/pubmed/34062822
http://dx.doi.org/10.3390/biomedicines9050499
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author Gao, Qi
Rhee, Claire
Maruyama, Masahiro
Li, Zhong
Shen, Huaishuang
Zhang, Ning
Utsunomiya, Takeshi
Huang, Elijah Ejun
Yao, Zhenyu
Bunnell, Bruce A.
Lin, Hang
Tuan, Rocky S.
Goodman, Stuart B.
author_facet Gao, Qi
Rhee, Claire
Maruyama, Masahiro
Li, Zhong
Shen, Huaishuang
Zhang, Ning
Utsunomiya, Takeshi
Huang, Elijah Ejun
Yao, Zhenyu
Bunnell, Bruce A.
Lin, Hang
Tuan, Rocky S.
Goodman, Stuart B.
author_sort Gao, Qi
collection PubMed
description Wear debris generated from the bearing surfaces of joint arthroplasties leads to acute and chronic inflammation, which is strongly associated with implant failure. Macrophages derived from monocytes recruited to the local tissues have a significant impact on bone healing and regeneration. Macrophages can adopt various functional phenotypes. While M1 macrophages are pro-inflammatory, M2 macrophages express factors important for tissue repair. Here, we established a 3D co-culture system to investigate how the immune system influences the osteogenic differentiation of mesenchymal stem cells (MSCs) in the presence of micron-sized particles. This system allowed for the simulation of an inflammatory reaction via the addition of Lipopolysaccharide-contaminated polyethylene particles (cPE) and the characterization of bone formation using micro-CT and gene and protein expression. Co-cultures of MSCs with M2 macrophages in the presence of cPE in a 3D environment resulted in the increased expression of osteogenic markers, suggesting facilitation of bone formation. In this model, the upregulation of M2 macrophage expression of immune-associated genes and cytokines contributes to enhanced bone formation by MSCs. This study elucidates how the immune system modulates bone healing in response to an inflammatory stimulus using a unique 3D culture system.
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spelling pubmed-81473322021-05-26 The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles Gao, Qi Rhee, Claire Maruyama, Masahiro Li, Zhong Shen, Huaishuang Zhang, Ning Utsunomiya, Takeshi Huang, Elijah Ejun Yao, Zhenyu Bunnell, Bruce A. Lin, Hang Tuan, Rocky S. Goodman, Stuart B. Biomedicines Communication Wear debris generated from the bearing surfaces of joint arthroplasties leads to acute and chronic inflammation, which is strongly associated with implant failure. Macrophages derived from monocytes recruited to the local tissues have a significant impact on bone healing and regeneration. Macrophages can adopt various functional phenotypes. While M1 macrophages are pro-inflammatory, M2 macrophages express factors important for tissue repair. Here, we established a 3D co-culture system to investigate how the immune system influences the osteogenic differentiation of mesenchymal stem cells (MSCs) in the presence of micron-sized particles. This system allowed for the simulation of an inflammatory reaction via the addition of Lipopolysaccharide-contaminated polyethylene particles (cPE) and the characterization of bone formation using micro-CT and gene and protein expression. Co-cultures of MSCs with M2 macrophages in the presence of cPE in a 3D environment resulted in the increased expression of osteogenic markers, suggesting facilitation of bone formation. In this model, the upregulation of M2 macrophage expression of immune-associated genes and cytokines contributes to enhanced bone formation by MSCs. This study elucidates how the immune system modulates bone healing in response to an inflammatory stimulus using a unique 3D culture system. MDPI 2021-05-01 /pmc/articles/PMC8147332/ /pubmed/34062822 http://dx.doi.org/10.3390/biomedicines9050499 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gao, Qi
Rhee, Claire
Maruyama, Masahiro
Li, Zhong
Shen, Huaishuang
Zhang, Ning
Utsunomiya, Takeshi
Huang, Elijah Ejun
Yao, Zhenyu
Bunnell, Bruce A.
Lin, Hang
Tuan, Rocky S.
Goodman, Stuart B.
The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
title The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
title_full The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
title_fullStr The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
title_full_unstemmed The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
title_short The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
title_sort effects of macrophage phenotype on osteogenic differentiation of mscs in the presence of polyethylene particles
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147332/
https://www.ncbi.nlm.nih.gov/pubmed/34062822
http://dx.doi.org/10.3390/biomedicines9050499
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