The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles
Wear debris generated from the bearing surfaces of joint arthroplasties leads to acute and chronic inflammation, which is strongly associated with implant failure. Macrophages derived from monocytes recruited to the local tissues have a significant impact on bone healing and regeneration. Macrophage...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147332/ https://www.ncbi.nlm.nih.gov/pubmed/34062822 http://dx.doi.org/10.3390/biomedicines9050499 |
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author | Gao, Qi Rhee, Claire Maruyama, Masahiro Li, Zhong Shen, Huaishuang Zhang, Ning Utsunomiya, Takeshi Huang, Elijah Ejun Yao, Zhenyu Bunnell, Bruce A. Lin, Hang Tuan, Rocky S. Goodman, Stuart B. |
author_facet | Gao, Qi Rhee, Claire Maruyama, Masahiro Li, Zhong Shen, Huaishuang Zhang, Ning Utsunomiya, Takeshi Huang, Elijah Ejun Yao, Zhenyu Bunnell, Bruce A. Lin, Hang Tuan, Rocky S. Goodman, Stuart B. |
author_sort | Gao, Qi |
collection | PubMed |
description | Wear debris generated from the bearing surfaces of joint arthroplasties leads to acute and chronic inflammation, which is strongly associated with implant failure. Macrophages derived from monocytes recruited to the local tissues have a significant impact on bone healing and regeneration. Macrophages can adopt various functional phenotypes. While M1 macrophages are pro-inflammatory, M2 macrophages express factors important for tissue repair. Here, we established a 3D co-culture system to investigate how the immune system influences the osteogenic differentiation of mesenchymal stem cells (MSCs) in the presence of micron-sized particles. This system allowed for the simulation of an inflammatory reaction via the addition of Lipopolysaccharide-contaminated polyethylene particles (cPE) and the characterization of bone formation using micro-CT and gene and protein expression. Co-cultures of MSCs with M2 macrophages in the presence of cPE in a 3D environment resulted in the increased expression of osteogenic markers, suggesting facilitation of bone formation. In this model, the upregulation of M2 macrophage expression of immune-associated genes and cytokines contributes to enhanced bone formation by MSCs. This study elucidates how the immune system modulates bone healing in response to an inflammatory stimulus using a unique 3D culture system. |
format | Online Article Text |
id | pubmed-8147332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81473322021-05-26 The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles Gao, Qi Rhee, Claire Maruyama, Masahiro Li, Zhong Shen, Huaishuang Zhang, Ning Utsunomiya, Takeshi Huang, Elijah Ejun Yao, Zhenyu Bunnell, Bruce A. Lin, Hang Tuan, Rocky S. Goodman, Stuart B. Biomedicines Communication Wear debris generated from the bearing surfaces of joint arthroplasties leads to acute and chronic inflammation, which is strongly associated with implant failure. Macrophages derived from monocytes recruited to the local tissues have a significant impact on bone healing and regeneration. Macrophages can adopt various functional phenotypes. While M1 macrophages are pro-inflammatory, M2 macrophages express factors important for tissue repair. Here, we established a 3D co-culture system to investigate how the immune system influences the osteogenic differentiation of mesenchymal stem cells (MSCs) in the presence of micron-sized particles. This system allowed for the simulation of an inflammatory reaction via the addition of Lipopolysaccharide-contaminated polyethylene particles (cPE) and the characterization of bone formation using micro-CT and gene and protein expression. Co-cultures of MSCs with M2 macrophages in the presence of cPE in a 3D environment resulted in the increased expression of osteogenic markers, suggesting facilitation of bone formation. In this model, the upregulation of M2 macrophage expression of immune-associated genes and cytokines contributes to enhanced bone formation by MSCs. This study elucidates how the immune system modulates bone healing in response to an inflammatory stimulus using a unique 3D culture system. MDPI 2021-05-01 /pmc/articles/PMC8147332/ /pubmed/34062822 http://dx.doi.org/10.3390/biomedicines9050499 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Gao, Qi Rhee, Claire Maruyama, Masahiro Li, Zhong Shen, Huaishuang Zhang, Ning Utsunomiya, Takeshi Huang, Elijah Ejun Yao, Zhenyu Bunnell, Bruce A. Lin, Hang Tuan, Rocky S. Goodman, Stuart B. The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles |
title | The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles |
title_full | The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles |
title_fullStr | The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles |
title_full_unstemmed | The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles |
title_short | The Effects of Macrophage Phenotype on Osteogenic Differentiation of MSCs in the Presence of Polyethylene Particles |
title_sort | effects of macrophage phenotype on osteogenic differentiation of mscs in the presence of polyethylene particles |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147332/ https://www.ncbi.nlm.nih.gov/pubmed/34062822 http://dx.doi.org/10.3390/biomedicines9050499 |
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