Cargando…

Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various...

Descripción completa

Detalles Bibliográficos
Autores principales: Babich, Olga, Sukhikh, Stanislav, Pungin, Artem, Astahova, Lidiya, Chupakhin, Evgeny, Belova, Daria, Prosekov, Alexander, Ivanova, Svetlana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147393/
https://www.ncbi.nlm.nih.gov/pubmed/34063290
http://dx.doi.org/10.3390/plants10050915
_version_ 1783697620830519296
author Babich, Olga
Sukhikh, Stanislav
Pungin, Artem
Astahova, Lidiya
Chupakhin, Evgeny
Belova, Daria
Prosekov, Alexander
Ivanova, Svetlana
author_facet Babich, Olga
Sukhikh, Stanislav
Pungin, Artem
Astahova, Lidiya
Chupakhin, Evgeny
Belova, Daria
Prosekov, Alexander
Ivanova, Svetlana
author_sort Babich, Olga
collection PubMed
description The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.
format Online
Article
Text
id pubmed-8147393
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81473932021-05-26 Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds Babich, Olga Sukhikh, Stanislav Pungin, Artem Astahova, Lidiya Chupakhin, Evgeny Belova, Daria Prosekov, Alexander Ivanova, Svetlana Plants (Basel) Brief Report The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites. MDPI 2021-05-02 /pmc/articles/PMC8147393/ /pubmed/34063290 http://dx.doi.org/10.3390/plants10050915 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Babich, Olga
Sukhikh, Stanislav
Pungin, Artem
Astahova, Lidiya
Chupakhin, Evgeny
Belova, Daria
Prosekov, Alexander
Ivanova, Svetlana
Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
title Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
title_full Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
title_fullStr Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
title_full_unstemmed Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
title_short Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds
title_sort evaluation of the conditions for the cultivation of callus cultures of hyssopus officinalis regarding the yield of polyphenolic compounds
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147393/
https://www.ncbi.nlm.nih.gov/pubmed/34063290
http://dx.doi.org/10.3390/plants10050915
work_keys_str_mv AT babicholga evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT sukhikhstanislav evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT punginartem evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT astahovalidiya evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT chupakhinevgeny evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT belovadaria evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT prosekovalexander evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds
AT ivanovasvetlana evaluationoftheconditionsforthecultivationofcallusculturesofhyssopusofficinalisregardingtheyieldofpolyphenoliccompounds