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Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism

In this study we evaluated whether single nucleotide polymorphisms (SNPs) in the genes encoding PTH, VDR, CYP24A1, and CYP27B1 were associated with mandibular retrognathism (MR). Samples from biologically-unrelated Brazilian patients receiving orthodontic treatment were included in this study. Pre-o...

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Autores principales: Küchler, Erika Calvano, Reis, Caio Luiz Bitencourt, Marañón-Vásquez, Guido, Nelson-Filho, Paulo, Matsumoto, Mírian Aiko Nakane, Stuani, Maria Bernadete Sasso, Oliveira, Maria Angélica Hueb de Menezes, Proff, Peter, Kirschneck, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147469/
https://www.ncbi.nlm.nih.gov/pubmed/34063310
http://dx.doi.org/10.3390/jpm11050369
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author Küchler, Erika Calvano
Reis, Caio Luiz Bitencourt
Marañón-Vásquez, Guido
Nelson-Filho, Paulo
Matsumoto, Mírian Aiko Nakane
Stuani, Maria Bernadete Sasso
Oliveira, Maria Angélica Hueb de Menezes
Proff, Peter
Kirschneck, Christian
author_facet Küchler, Erika Calvano
Reis, Caio Luiz Bitencourt
Marañón-Vásquez, Guido
Nelson-Filho, Paulo
Matsumoto, Mírian Aiko Nakane
Stuani, Maria Bernadete Sasso
Oliveira, Maria Angélica Hueb de Menezes
Proff, Peter
Kirschneck, Christian
author_sort Küchler, Erika Calvano
collection PubMed
description In this study we evaluated whether single nucleotide polymorphisms (SNPs) in the genes encoding PTH, VDR, CYP24A1, and CYP27B1 were associated with mandibular retrognathism (MR). Samples from biologically-unrelated Brazilian patients receiving orthodontic treatment were included in this study. Pre-orthodontic lateral cephalograms were used to determine the phenotype. Patients with a retrognathic mandible were selected as cases and those with an orthognathic mandible were selected as controls. Genomic DNA was used for genotyping analysis of SNPs in PTH (rs694, rs6256, and rs307247), VDR (rs7975232), CYP24A1 (rs464653), and CYP27B1 (rs927650). Chi-squared or Fisher’s tests were used to compare genotype and allele distribution among groups. Haplotype analysis was performed for the SNPs in PTH. The established alpha was p < 0.05. Multifactor dimensionality reduction (MDR) was used to identify SNP–SNP interactions. A total of 48 (22 males and 26 females) MR and 43 (17 males and 26 females) controls were included. The linear mandibular and the angular measurements were statistically different between MR and controls (p < 0.05). In the genotype and allele distribution analysis, the SNPs rs694, rs307247, and rs464653 were associated with MR (p < 0.05). MDR analyses predicted the best interaction model for MR was rs694–rs927650, followed by rs307247–rs464653–rs927650. Some haplotypes in the PTH gene presented statistical significance. Our results suggest that SNPs in PTH, VDR, CYP24A1, and CYP27B1 genes are associated with the presence of mandibular retrognathism.
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spelling pubmed-81474692021-05-26 Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism Küchler, Erika Calvano Reis, Caio Luiz Bitencourt Marañón-Vásquez, Guido Nelson-Filho, Paulo Matsumoto, Mírian Aiko Nakane Stuani, Maria Bernadete Sasso Oliveira, Maria Angélica Hueb de Menezes Proff, Peter Kirschneck, Christian J Pers Med Article In this study we evaluated whether single nucleotide polymorphisms (SNPs) in the genes encoding PTH, VDR, CYP24A1, and CYP27B1 were associated with mandibular retrognathism (MR). Samples from biologically-unrelated Brazilian patients receiving orthodontic treatment were included in this study. Pre-orthodontic lateral cephalograms were used to determine the phenotype. Patients with a retrognathic mandible were selected as cases and those with an orthognathic mandible were selected as controls. Genomic DNA was used for genotyping analysis of SNPs in PTH (rs694, rs6256, and rs307247), VDR (rs7975232), CYP24A1 (rs464653), and CYP27B1 (rs927650). Chi-squared or Fisher’s tests were used to compare genotype and allele distribution among groups. Haplotype analysis was performed for the SNPs in PTH. The established alpha was p < 0.05. Multifactor dimensionality reduction (MDR) was used to identify SNP–SNP interactions. A total of 48 (22 males and 26 females) MR and 43 (17 males and 26 females) controls were included. The linear mandibular and the angular measurements were statistically different between MR and controls (p < 0.05). In the genotype and allele distribution analysis, the SNPs rs694, rs307247, and rs464653 were associated with MR (p < 0.05). MDR analyses predicted the best interaction model for MR was rs694–rs927650, followed by rs307247–rs464653–rs927650. Some haplotypes in the PTH gene presented statistical significance. Our results suggest that SNPs in PTH, VDR, CYP24A1, and CYP27B1 genes are associated with the presence of mandibular retrognathism. MDPI 2021-05-02 /pmc/articles/PMC8147469/ /pubmed/34063310 http://dx.doi.org/10.3390/jpm11050369 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Küchler, Erika Calvano
Reis, Caio Luiz Bitencourt
Marañón-Vásquez, Guido
Nelson-Filho, Paulo
Matsumoto, Mírian Aiko Nakane
Stuani, Maria Bernadete Sasso
Oliveira, Maria Angélica Hueb de Menezes
Proff, Peter
Kirschneck, Christian
Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism
title Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism
title_full Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism
title_fullStr Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism
title_full_unstemmed Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism
title_short Parathyroid Hormone Gene and Genes Involved in the Maintenance of Vitamin D Levels Association with Mandibular Retrognathism
title_sort parathyroid hormone gene and genes involved in the maintenance of vitamin d levels association with mandibular retrognathism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147469/
https://www.ncbi.nlm.nih.gov/pubmed/34063310
http://dx.doi.org/10.3390/jpm11050369
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