Characterisation of Dichelobacter nodosus on Misshapen and Damaged Ovine Feet: A Longitudinal Study of Four UK Sheep Flocks

SIMPLE SUMMARY: The bacterium Dichelobacter nodosus is the cause of footrot in sheep: a painful, contagious foot disease. Some sheep may be more susceptible to carrying D. nodosus on their feet, despite showing no signs of disease, and pose a risk to flock health and welfare. This study investigated whether misshapen or damaged feet (poor hoof conformation) were more likely to have D. nodosus present and in greater quantities than feet in good condition. Eighty-five ewes from four flocks were examined three times, on average, across a 12-month period. Poor hoof conformation traits were observed in over 92% of foot observations. Feet with poor sole and heel conformation were more likely to have higher quantities of D. nodosus than those in good conformation. Furthermore, on feet positive for D. nodosus, wall overgrowth contributed towards higher D. nodosus load. We highlight feet with aspects of poor conformation traits to covertly harbour D. nodosus. These feet could transmit D. nodosus to other feet of sheep. Increasing our understanding of D. nodosus infection is crucial in helping farmers improve management practices to reduce footrot. ABSTRACT: Dichelobacter nodosus is the causal agent of ovine footrot, a contagious disease of welfare and economic concern worldwide. Damaged feet may be subclinical carriers of D. nodosus and covertly spread infection. Accordingly, we evaluated the risk of misshapen and damaged feet on D. nodosus presence and load in four commercial UK sheep flocks. Foot-level observations and swabs (n = 972) were collected from ewes (n = 85) over 12 months. On average, ewes were sampled three times. Feet were inspected for disease and scored (good/poor) for three hoof conformation traits (sole and heel, wall, and wall overgrowth). Swabs were analysed for presence and load of D. nodosus, and mixed models were constructed. Poor hoof conformation traits were present in 92.5% of foot-level observations. Feet with poor sole and heel conformation were more likely to have higher D. nodosus loads (β = 0.19, 95% CI: 0.04–0.35) than those with good conformation. Furthermore, on feet positive for D. nodosus, wall overgrowth was associated with higher D. nodosus loads (β = 0.27, 95% CI: 0.01–0.52). Feet with aspects of poor conformation covertly harbour D. nodosus and are a source of infection. Flock management should be guided by hoof conformation to reduce disease challenge.