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MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis
BACKGROUND: This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis. MATERIALS & METHODS: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values wer...
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Future Science Ltd
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147753/ https://www.ncbi.nlm.nih.gov/pubmed/34046191 http://dx.doi.org/10.2144/fsoa-2021-0006 |
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| author | Kiyoshima, Chihiro Shirasu, Naoto Urushiyama, Daichi Fukagawa, Satoshi Hirakawa, Toyofumi Yoshikawa, Kenichi Izuchi, Daisuke Miyata, Kohei Kurakazu, Masamitsu Yotsumoto, Fusanori Hiromatsu, Kenji Nomiyama, Makoto Eiji, Ohta Hirose, Shinichi Ogura, Yoshitoshi Hayashi, Tetsuya Hata, Kenichiro Nabeshima, Kazuki Yasunaga, Shin'ichiro Miyamoto, Shingo |
| author_facet | Kiyoshima, Chihiro Shirasu, Naoto Urushiyama, Daichi Fukagawa, Satoshi Hirakawa, Toyofumi Yoshikawa, Kenichi Izuchi, Daisuke Miyata, Kohei Kurakazu, Masamitsu Yotsumoto, Fusanori Hiromatsu, Kenji Nomiyama, Makoto Eiji, Ohta Hirose, Shinichi Ogura, Yoshitoshi Hayashi, Tetsuya Hata, Kenichiro Nabeshima, Kazuki Yasunaga, Shin'ichiro Miyamoto, Shingo |
| author_sort | Kiyoshima, Chihiro |
| collection | PubMed |
| description | BACKGROUND: This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis. MATERIALS & METHODS: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc’s criteria. RESULTS: The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation. CONCLUSION: miR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis. |
| format | Online Article Text |
| id | pubmed-8147753 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Future Science Ltd |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-81477532021-05-26 MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis Kiyoshima, Chihiro Shirasu, Naoto Urushiyama, Daichi Fukagawa, Satoshi Hirakawa, Toyofumi Yoshikawa, Kenichi Izuchi, Daisuke Miyata, Kohei Kurakazu, Masamitsu Yotsumoto, Fusanori Hiromatsu, Kenji Nomiyama, Makoto Eiji, Ohta Hirose, Shinichi Ogura, Yoshitoshi Hayashi, Tetsuya Hata, Kenichiro Nabeshima, Kazuki Yasunaga, Shin'ichiro Miyamoto, Shingo Future Sci OA Research Article BACKGROUND: This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis. MATERIALS & METHODS: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc’s criteria. RESULTS: The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation. CONCLUSION: miR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis. Future Science Ltd 2021-02-15 /pmc/articles/PMC8147753/ /pubmed/34046191 http://dx.doi.org/10.2144/fsoa-2021-0006 Text en © 2021 Chihiro Kiyoshima https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) |
| spellingShingle | Research Article Kiyoshima, Chihiro Shirasu, Naoto Urushiyama, Daichi Fukagawa, Satoshi Hirakawa, Toyofumi Yoshikawa, Kenichi Izuchi, Daisuke Miyata, Kohei Kurakazu, Masamitsu Yotsumoto, Fusanori Hiromatsu, Kenji Nomiyama, Makoto Eiji, Ohta Hirose, Shinichi Ogura, Yoshitoshi Hayashi, Tetsuya Hata, Kenichiro Nabeshima, Kazuki Yasunaga, Shin'ichiro Miyamoto, Shingo MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| title | MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| title_full | MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| title_fullStr | MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| title_full_unstemmed | MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| title_short | MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| title_sort | micrornas mir-4535 and mir-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis |
| topic | Research Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147753/ https://www.ncbi.nlm.nih.gov/pubmed/34046191 http://dx.doi.org/10.2144/fsoa-2021-0006 |
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