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Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)

Babesia bovis is a widely-spread tick-borne hemoparasite of cattle with major economic and animal welfare consequences. Rhipicephalus (Boophilus) annulatus is a one-host tick which transmits bovine babesiosis in the Middle East and Africa. Laboratory rearing of ixodid ticks is essential for the inve...

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Autores principales: Tirosh-Levy, Sharon, Roth, Asael, Leibovich, Binyamin, Fleiderovitz, Ludmila, Frid, Ohad, Yasur-Landau, Daniel, Wolkomirskyi, Ricardo, Mazuz, Monica L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147767/
https://www.ncbi.nlm.nih.gov/pubmed/34063706
http://dx.doi.org/10.3390/pathogens10050554
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author Tirosh-Levy, Sharon
Roth, Asael
Leibovich, Binyamin
Fleiderovitz, Ludmila
Frid, Ohad
Yasur-Landau, Daniel
Wolkomirskyi, Ricardo
Mazuz, Monica L.
author_facet Tirosh-Levy, Sharon
Roth, Asael
Leibovich, Binyamin
Fleiderovitz, Ludmila
Frid, Ohad
Yasur-Landau, Daniel
Wolkomirskyi, Ricardo
Mazuz, Monica L.
author_sort Tirosh-Levy, Sharon
collection PubMed
description Babesia bovis is a widely-spread tick-borne hemoparasite of cattle with major economic and animal welfare consequences. Rhipicephalus (Boophilus) annulatus is a one-host tick which transmits bovine babesiosis in the Middle East and Africa. Laboratory rearing of ixodid ticks is essential for the investigation on ticks or tick-borne diseases. Establishing a tick colony in the laboratory usually originates from ticks harvested in the field, which may be naturally infected with various pathogens. This especially applies to carriage of B. bovis as it is highly prevalent in endemic areas and is transmitted transovarially in ticks. Here, we describe the use of diminazene aceturate (Berenil) in order to establish laboratory colonies of Babesia-free R. annulatus, from ticks collected in the field. Ticks collected in the field were kept until oviposition and hatched larvae were introduced to naïve calves, which led to infection of the calves with B. bovis. Calves were then treated with diminazene aceturate several times until the engorged ticks dropped. The eggs and larvae collected from these ticks were parasite-free, as demonstrated both by infection of splenectomized calves and by PCR. This suggested protocol is a useful tool to create parasite-free tick colony and may, theoretically, also be beneficial to reduce parasite circulation in the field, although not recommended, as resistance to diamenizene aceturate might develop.
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spelling pubmed-81477672021-05-26 Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil) Tirosh-Levy, Sharon Roth, Asael Leibovich, Binyamin Fleiderovitz, Ludmila Frid, Ohad Yasur-Landau, Daniel Wolkomirskyi, Ricardo Mazuz, Monica L. Pathogens Article Babesia bovis is a widely-spread tick-borne hemoparasite of cattle with major economic and animal welfare consequences. Rhipicephalus (Boophilus) annulatus is a one-host tick which transmits bovine babesiosis in the Middle East and Africa. Laboratory rearing of ixodid ticks is essential for the investigation on ticks or tick-borne diseases. Establishing a tick colony in the laboratory usually originates from ticks harvested in the field, which may be naturally infected with various pathogens. This especially applies to carriage of B. bovis as it is highly prevalent in endemic areas and is transmitted transovarially in ticks. Here, we describe the use of diminazene aceturate (Berenil) in order to establish laboratory colonies of Babesia-free R. annulatus, from ticks collected in the field. Ticks collected in the field were kept until oviposition and hatched larvae were introduced to naïve calves, which led to infection of the calves with B. bovis. Calves were then treated with diminazene aceturate several times until the engorged ticks dropped. The eggs and larvae collected from these ticks were parasite-free, as demonstrated both by infection of splenectomized calves and by PCR. This suggested protocol is a useful tool to create parasite-free tick colony and may, theoretically, also be beneficial to reduce parasite circulation in the field, although not recommended, as resistance to diamenizene aceturate might develop. MDPI 2021-05-03 /pmc/articles/PMC8147767/ /pubmed/34063706 http://dx.doi.org/10.3390/pathogens10050554 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tirosh-Levy, Sharon
Roth, Asael
Leibovich, Binyamin
Fleiderovitz, Ludmila
Frid, Ohad
Yasur-Landau, Daniel
Wolkomirskyi, Ricardo
Mazuz, Monica L.
Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
title Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
title_full Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
title_fullStr Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
title_full_unstemmed Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
title_short Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
title_sort establishing babesia bovis-free tick colony following treatment of the host with diminazene aceturate (berenil)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147767/
https://www.ncbi.nlm.nih.gov/pubmed/34063706
http://dx.doi.org/10.3390/pathogens10050554
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