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Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi

Woody fruit which stay on ornamental plants for a long time may present a risk of infection to other organisms due to the presence of pathogens on their surface. We compared the microbe communities on the fruit surfaces of garden ornamental Gardenia thunbergia Thunb. with those on other surfaces in...

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Autores principales: Steudel, Bastian, Baijnath, Himansu, Schwedt, Thorben, Schmitt, Armin Otto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147784/
https://www.ncbi.nlm.nih.gov/pubmed/34064327
http://dx.doi.org/10.3390/pathogens10050555
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author Steudel, Bastian
Baijnath, Himansu
Schwedt, Thorben
Schmitt, Armin Otto
author_facet Steudel, Bastian
Baijnath, Himansu
Schwedt, Thorben
Schmitt, Armin Otto
author_sort Steudel, Bastian
collection PubMed
description Woody fruit which stay on ornamental plants for a long time may present a risk of infection to other organisms due to the presence of pathogens on their surface. We compared the microbe communities on the fruit surfaces of garden ornamental Gardenia thunbergia Thunb. with those on other surfaces in the study region. As Gardenia fruit contain antifungal substances, the focus of this study was on the fungal communities that exist thereon. We used Illumina sequencing to identify Amplicon Sequence Variants (ASV) of the internal transcribed spacer 2 (ITS2) of the ribosomal RNA. The microbial communities of the Gardenia fruit are distinct from the communities from the surrounding environments, indicating a specialized microhabitat. We employed clustering methods to position unidentified ASVs relative to known ASVs. We identified a total of 56 ASVs representing high risk fungal species as putative plant pathogens exclusively found on the fruit of Gardenia. Additionally, we found several ASVs representing putative animal or human pathogens. Those pathogens were distributed over distinct fungi clades. The infection risk of the high diversity of putative pathogens represented on the Gardenia fruit needs to be elucidated in further investigations.
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spelling pubmed-81477842021-05-26 Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi Steudel, Bastian Baijnath, Himansu Schwedt, Thorben Schmitt, Armin Otto Pathogens Article Woody fruit which stay on ornamental plants for a long time may present a risk of infection to other organisms due to the presence of pathogens on their surface. We compared the microbe communities on the fruit surfaces of garden ornamental Gardenia thunbergia Thunb. with those on other surfaces in the study region. As Gardenia fruit contain antifungal substances, the focus of this study was on the fungal communities that exist thereon. We used Illumina sequencing to identify Amplicon Sequence Variants (ASV) of the internal transcribed spacer 2 (ITS2) of the ribosomal RNA. The microbial communities of the Gardenia fruit are distinct from the communities from the surrounding environments, indicating a specialized microhabitat. We employed clustering methods to position unidentified ASVs relative to known ASVs. We identified a total of 56 ASVs representing high risk fungal species as putative plant pathogens exclusively found on the fruit of Gardenia. Additionally, we found several ASVs representing putative animal or human pathogens. Those pathogens were distributed over distinct fungi clades. The infection risk of the high diversity of putative pathogens represented on the Gardenia fruit needs to be elucidated in further investigations. MDPI 2021-05-04 /pmc/articles/PMC8147784/ /pubmed/34064327 http://dx.doi.org/10.3390/pathogens10050555 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Steudel, Bastian
Baijnath, Himansu
Schwedt, Thorben
Schmitt, Armin Otto
Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
title Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
title_full Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
title_fullStr Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
title_full_unstemmed Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
title_short Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
title_sort microeukaryotic communities on the fruit of gardenia thunbergia thunb. with a focus on pathogenic fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147784/
https://www.ncbi.nlm.nih.gov/pubmed/34064327
http://dx.doi.org/10.3390/pathogens10050555
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