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Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics

Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool...

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Autores principales: Stupar, Petar, Podolski-Renić, Ana, Villalba, Maria Ines, Dragoj, Miodrag, Jovanović Stojanov, Sofija, Pešić, Milica, Kasas, Sandor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147836/
https://www.ncbi.nlm.nih.gov/pubmed/34064439
http://dx.doi.org/10.3390/medicina57050446
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author Stupar, Petar
Podolski-Renić, Ana
Villalba, Maria Ines
Dragoj, Miodrag
Jovanović Stojanov, Sofija
Pešić, Milica
Kasas, Sandor
author_facet Stupar, Petar
Podolski-Renić, Ana
Villalba, Maria Ines
Dragoj, Miodrag
Jovanović Stojanov, Sofija
Pešić, Milica
Kasas, Sandor
author_sort Stupar, Petar
collection PubMed
description Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.
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spelling pubmed-81478362021-05-26 Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics Stupar, Petar Podolski-Renić, Ana Villalba, Maria Ines Dragoj, Miodrag Jovanović Stojanov, Sofija Pešić, Milica Kasas, Sandor Medicina (Kaunas) Article Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability. MDPI 2021-05-04 /pmc/articles/PMC8147836/ /pubmed/34064439 http://dx.doi.org/10.3390/medicina57050446 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stupar, Petar
Podolski-Renić, Ana
Villalba, Maria Ines
Dragoj, Miodrag
Jovanović Stojanov, Sofija
Pešić, Milica
Kasas, Sandor
Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
title Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
title_full Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
title_fullStr Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
title_full_unstemmed Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
title_short Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
title_sort nano-motion analysis for rapid and label free assessing of cancer cell sensitivity to chemotherapeutics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147836/
https://www.ncbi.nlm.nih.gov/pubmed/34064439
http://dx.doi.org/10.3390/medicina57050446
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